人原发性食管癌组织中多种抑癌基因的研究

The multiple tumor suppressor genes in humanesophageal cancer

应用ＰＣＲ扩增与直接测序技术，分析１０例食管癌和癌旁组织标本中多个抑癌基因ｐ５３、Ｒｂ、ＡＰＣ和ＭＣＣ的突变与丢失。结果：１０例中６例有ｐ５３基因突变；５例有Ｒｂ基因改变；３例有ＡＰＣ改变；３例有ＭＣＣ基因改变；８例食管癌标本中至少有一种抑癌基因有改变；６例至少有两种或多种抑癌基因改变，１例癌旁组织中也有两个抑癌基因改变。结果表明，食管癌癌变与多个抑癌基因变化的累积有关。

he mutation and deletion of the multiple tumorsuppressor genes, including p53, Rb, APC and MCCin the same tissue of humanesophageal cancer (EC)and adjacent non-tumor were analysed by PCR am-plification and direct sequencing. In 10 cases of EC 6were found mutationsof p53 gene , 5 were found ab-normiality of Rb gene , 3 were found mutation ofAPC gene, 3 were found mutation of MCC gene, 8were found atleration abnormality of tumorsuppressor genes Rb, p53, APC and MCC, 6 werefound two or more abnormality of the tumorsuppressor genes. The results indicated that the altera-tions in multiple of tumor suppressor genes were re-lated to carcinogenesis of human esophageal cancer.