石榴皮多酚提取物降血脂效果的实验研究

EXPERIMENTAL STUDY ON HYPOLIPIDEMIC EFFECT OF POLYPHENOLIC EXTRACT FROM POMEGRANATE PEEL

目的提取和富集石榴皮中的多酚类物质,并探讨其降血脂效果。方法采用乙酸乙酯萃取石榴皮粗提取物中的多酚类物质并富集,以高脂饲料喂养雄性SD大鼠建立高脂血症模型,观察饲料补充提取物喂养28d对大鼠血脂和肝脂水平的影响。结果石榴皮粗提取物经进一步分离提取后,多酚类物质得到了富集。高脂组大鼠经多酚提取物饲养后,其血清TC、TG、LDL-C、FFA和肝TC、TG、FFA比高脂对照组分别减少42.4%、58.5%、48.9%、20.6%和32.6%、11.9%、25.5%。部分作用有强于石榴皮粗提取物的趋势,而且还具有提高血清高密度脂蛋白胆固醇(HDL-C)水平的作用。结论石榴皮多酚提取物具有降低血脂和肝脂的作用,有效成分有待进一步分析。

Objective To extract and concentrate the polyphenols from pomegranate peel and to observe the effect of lowering lipids in hyperlipidemic rats. Methods The polyphenols contained in pomegranate peel crude extract were isolated and concentrated using ethyl acetate. Male Sprague-Dawley rats were fed a high-fat diet to induce hyperlipidemia and pomegranate peel crude extract or pomegranate peel polyphenolic extract was supplemented respectively for 28 days. Serum and hepatic lipids were examined. Results Pomegranate peel polyphenolic extract was isolated and concentrated. Serum cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C), free fatty acid(FFA) levels as well as liver TC, TG, and FFA levels were 42.4%,58.5%,48.9%,20.6%,and 32.6%,11.9%,25.5% less respectively in hyperlipidemic rats supplemented with polyphenolic extract than in hyperlipidemic controls (P<0.05 or P<0.01).Some of the lipid-lowering effect of pomegranate peel polyphenolic extract showed better tendency than crude extract. Conclusion Pomegranate peel polyphenolic extract is effective in lowering serum and hepatic lipids. Further study is necessary to determine the critical polyphenolic component in the extract.