摘要
应用流式细胞分析仪检测乳腺癌(42例)、乳腺纤维腺瘤(10例)、乳腺囊性增生病(10例)及正常乳腺组织(10例)的细胞DNA含量。42例乳腺癌标本中,26例(62%)为异倍体DNA;乳腺囊性增生病和正常乳腺组织均为二倍体DNA。乳腺癌标本均行雌激素受体(ER)测定,11例(26%)为阴性。同时对所有乳腺癌进行有关临床资料和病理分析,发现乳腺癌DNA含量与病人年龄及ER状态差异均有显著性意义(P<0.05)。乳腺肿瘤细胞周期分析发现异倍体癌S期细胞比率最高。通过乳腺癌细胞DNA含量及ER测定,计算细胞增殖周期中各期细胞比率,可以判断乳腺癌生物学特性,为患者预后的评估提供客观、定量和可重复性参数,弥补形态学检查的不足,并对术后辅助治疗有指导意义。
Abstract Cellular DNA content of 72 samples was analyzed by flow
cytometry. The results showed that20 benign tumors and l0 normal breast tissue specimens
were found to be DNA diploidy. 26(62%) of 42 cases of breast cancer were DNA aneuploidy and
the remainders were DNA diploidy. The median s- phase cell percentage was significantly
higher in aneuploid tumors (28 %) thandiploid tumors (13%)and benign tumors (4%). Estrogen
receptors were also investigated in 42 breastcancer specimens. There were 31 ER positive
(74%) cases and 11 negative(26%) cases. The aneuploid DNA content was usually associated
with lack of estrogen receptors. DNA con-tent correlated significantly with age and ER status.
The results suggest that flow cytometric analysis of aillular DNA content of breast cancer and
status of estrogen receptors can provide an objective quantitative and reproducible new
parameter for predicting prognosis of breast cancer.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1994年第6期461-464,共4页
Chinese Journal of Oncology