期刊文献+

高效液相色谱法测定吲达帕胺的血药浓度 被引量:8

Determination of indapamide in human plasma by HPLC method
下载PDF
导出
摘要 目的建立测定吲达帕胺血药浓度的方法。方法选择以乙腈(pH=2.8)磷酸缓冲液(32∶68)为流动相,格列吡嗪为内标,血浆样品以重蒸乙醚为提取溶剂,经DiamonsilC18(150mm×4.6mm,5μm)柱分离后,在紫外吸收波长240nm处进样40μL检测。结果吲达帕胺血浆样品在25~500ng/mL的范围内线性关系良好(r=0.9996,n=6),绝对回收率>67%,相对回收率>92%日内日间的RSD<6%。结论本法简便、准确、灵敏、重现性好,可用于吲达帕胺的药物动力学的研究。 OBJECTIVE To develop a method for determination of indapamide in plasma. METHODS RP-HPLC was applied to quantitative analysis. Plasma samples were extracted with ethylether. 40μl of extraction was injected and separated by Diamonsil C_ 18 (150mm*4.6mm,5μm) column. The mobile phase was composed of acetonitrile-(pH=2.8)phosphoric acid buffer (32∶68). glipizide was used as internal standard. The UV detective wavelength was 240nm for indapamide. RESULTS A good linearity within the range of 25-500ng.mL -1 ( r =0.9996) for indapamide was obtained. The absolute recovery was higher than 67% and the relative recovery was higher than 92%. The inter-day and intra-day RSD were less than 6%. CONCLUSION This method is simple, accurate, sensitive and reproducible.
出处 《中国现代应用药学》 CAS CSCD 北大核心 2005年第3期231-232,共2页 Chinese Journal of Modern Applied Pharmacy
关键词 吲达帕胺 高效液相 血药浓度 indapamide HPLC plasma concentration
  • 相关文献

参考文献4

  • 1陈端霞,游凯,傅如南,严晓伟,方圻.人血清中吲达胺的高效液相色谱法测定[J].中国医学科学院学报,1990,12(4):286-289. 被引量:8
  • 2Zendelovska D, Stafilov T, Stefova M. Optimization of a solid-phase extraction method for determination of indapamide in biological fluids using high-performance liquid chromatography[ J ]. J Chromatogr B Analyt Technol Biomed Life Sci, 2003,788 ( 1 ) :199.
  • 3Grebow PE, Treitman JA, Barry EP,et al. Pharmacokinetics and biovalability of indapamide A new antihypertensive drug [J]. Eur J Clin Pharmacol, 1982,22(4) :295.
  • 4Schiavi P, Jochemsen R, Guez D. Pharmacokinetics of sustained and immediate release formulations of indapamide after single and repeated oral administration in healthy volunteers [J].Fundam Clin Pharmacol, 2000,14 ( 2 ) : 139.

共引文献7

同被引文献2148

引证文献8

二级引证文献11

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部