摘要
目的建立测定吲达帕胺血药浓度的方法。方法选择以乙腈(pH=2.8)磷酸缓冲液(32∶68)为流动相,格列吡嗪为内标,血浆样品以重蒸乙醚为提取溶剂,经DiamonsilC18(150mm×4.6mm,5μm)柱分离后,在紫外吸收波长240nm处进样40μL检测。结果吲达帕胺血浆样品在25~500ng/mL的范围内线性关系良好(r=0.9996,n=6),绝对回收率>67%,相对回收率>92%日内日间的RSD<6%。结论本法简便、准确、灵敏、重现性好,可用于吲达帕胺的药物动力学的研究。
OBJECTIVE To develop a method for determination of indapamide in plasma. METHODS RP-HPLC was applied to quantitative analysis. Plasma samples were extracted with ethylether. 40μl of extraction was injected and separated by Diamonsil C_ 18 (150mm*4.6mm,5μm) column. The mobile phase was composed of acetonitrile-(pH=2.8)phosphoric acid buffer (32∶68). glipizide was used as internal standard. The UV detective wavelength was 240nm for indapamide. RESULTS A good linearity within the range of 25-500ng.mL -1 ( r =0.9996) for indapamide was obtained. The absolute recovery was higher than 67% and the relative recovery was higher than 92%. The inter-day and intra-day RSD were less than 6%. CONCLUSION This method is simple, accurate, sensitive and reproducible.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2005年第3期231-232,共2页
Chinese Journal of Modern Applied Pharmacy