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利用SDS-PAGE鉴定转基因小麦HMW-GS的表达类型和后代遗传 被引量：3

The Genotyes and Inheritance of High-Molecular-Weight Glutenin Subunits(HMW-GS) in Transgenic Wheat by SDS-PAGE
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摘要 采用SDS 聚丙烯酰胺凝胶电泳(SDS PAGE)在蛋白质水平上对T2～T5代小麦转基因株系高分子量谷蛋白亚基(HMW GS)的遗传表达及其分离进行了鉴定和分析。结果发现,外源1Dx5和1Dy10基因在T2代部分株系中表达;在有的株系中出现了原亚基(8亚基)的缺失或沉默和新亚基(暂定8 )的产生,因而检测出2+5+10+12,2+5+7+8 +10+12,5+7+10,2+7+10+12的多种类型。对2+5+10+12型株系进行了多代跟踪分析,发现其在T2～T5代陆续发生分离,存在于不同单株、不同单穗的子粒和同一单粒后代之间。经筛选,获得了天然不存在的亚基类型为2+5+10+12,2+10+12,2+5+12等稳定表达的种质创新株系。 The genotypes and inheritance of High-Molecular-Weight Glutenin Subunits (HMW-GS) in T_2-T_5 generations transgenic wheat plants were conduced by SDS-PAGE. The results showed that the exogenous 1Dx5 and 1Dy10 genes expressed in some plants in T_2 population, and in some plants caused some endogenous genes to be null and a new subunit to appear. Many genotypes such as 2+5+7+10+12, 2+5+7+8~*+10+12, 5+7+10,2+7+10+12,therefore, were picked out. The lines with 2+5+10+12 continue to separate from T_2 to T_5 in offspring populations of different plants, different spikes form one plant or different grains form one spike. So we get some stably expressed novel wheat lines with 2+5+10+12, 2+10+12, 2+5+12, which do not exist in nature were obtained.
作者 杨凤萍 梁荣奇 陈绪清 韩立新 张晓东
机构地区 北京农业生物技术研究中心
出处 《华北农学报》 CSCD 北大核心 2005年第2期1-4,共4页 Acta Agriculturae Boreali-Sinica
基金 国家科技部植物基因工程产业化招标项目(J2000-B-017)
关键词 SDS-PAGE HMW-GS 转基因小麦 SDS-聚丙烯酰胺凝胶电泳 类型 后代 鉴定 高分子量谷蛋白亚基 蛋白质水平 遗传表达 跟踪分析 稳定表达 新株系 分离 2型 单株 子粒 种质 穗 Transgenic Wheat HMW-GS SDS-PAGE Separation
分类号 S512.1 [农业科学—作物学] Q51 [生物学—生物化学]
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参考文献16

  • 1张晓东,李冬梅,徐文英,蒋有绎,胡道芬,韩立新.用基因枪将除草剂Basta抗性基因与小麦HMW谷蛋白亚基基因导入小麦获得转基因植株[J].华北农学报,1997,12(1):133-136. 被引量：32
  • 2华志华,朱雪峰,林鸿生,高振宇,钱前,颜美仙,黄大年.基因枪转化获得的转基因水稻中外源基因整合与表达规律研究[J].Acta Genetica Sinica,2001,28(11):1012-1018. 被引量：22
  • 3张晓东,梁荣奇,陈绪清,杨凤萍,张立全.优质HMW谷蛋白亚基转基因小麦的获得及其遗传稳定性和品质性状分析[J].科学通报,2003,48(5):474-479. 被引量：42
  • 4Payne P I, Law C N, Mudd E E. Control by homologous group chromosomes of the HMW-GS,a major protein of wheat endosperm [J]. Thero Appl Genet, 1980, 58: 113-120.
  • 5Thomopson R, Harberd N P, Flavell R B. Characterization of the multigene family coding for HMW-GS in wheat using cDNA clones[J]. Thero Appl Genet, 1983, 67: 87-96.
  • 6Harberd N P, Bartels D, Thompson R D. DNA restriction fragment variation in gene family encoding HMW-GS of wheat[J]. Biochem Genet, 1986, 24: 579-596.
  • 7Ford J, Nalpica J M, Halford N G. The nucleotide sequence of a HMW-GS gene located on chromosome 1A of wheat (Triticum aestivum L.)[J]. Nucleic Acids Res, 1985, 13: 6817-6832.
  • 8Payne P I, Lawrece G J. Catalogue of alleles for the complex gene loci,Glu-A1, Glu-B1 and Glu-D1 which code for HMW-GS in hexaoloid wheat[J]. Cereal Research Communication,1983, 11: 29-35.
  • 9刘广田 徐明辉.普通小麦胚乳谷蛋白亚基的遗传研究 I 高分子量谷蛋白亚基变异的多样性及其在F1代的遗传行为[J].中国农业科学,1988,(7):13-17.
  • 10Alvarez M L, Guelman S, Halford N G. Sliencing of HMW glutenins in transgenic wheat expressing extra HMW subunits[J]. Thero Appl Genet, 2000, 100: 319-327.

二级参考文献30

  • 1张晓东,李冬梅,徐文英,蒋有绎,胡道芬,韩立新.用基因枪将除草剂Basta抗性基因与小麦HMW谷蛋白亚基基因导入小麦获得转基因植株[J].华北农学报,1997,12(1):133-136. 被引量：32
  • 2刘广田 许明辉.普通小麦胚乳高分子量谷蛋白亚基的变异和遗传[J].中国农业科学,1988,21(1):56-58.
  • 3[1]Payne P I, Corfield K G, Holt L M. Correlation between the inheritance of certain HMW-GS and bread wheat making quality in progenies of six crosses of bread wheat. J Sci Food Agri, 1981, 32: 51 ～ 60
  • 4[2]Lorenzo A, Kronstad W E, Vieira G E. Relationships between HMW-GS and loaf volume in wheat as measured by the SDS sedimentation test. Crop Sci, 1987, 27: 253 ～ 257
  • 5[3]Huang D Y, Kham K. Quantitative determination of high molecular weight glutenin subunits of hard red spring wheat by SDS- PAGE Ⅱ. Quantitative effects of individual subunits on breadmaking quality characteristics. Cereal Chem, 1997, 74(6): 786～790
  • 6[4]Payne P I, Holt L M, Worland A G. Structure and genetical studies on the HMW-GS:Ⅲ. Telocentric mapping of the subunits genes on the long arm of the homoeologous groupⅠchromosomes. Thero Appl Genet, 1982, 63: 129～138
  • 7[5]Thompson R, Harberd N P, Flavell R B. Characterization of the multigene familycoding for HMW-GS in wheat using cDNA clones. Thero Appl Genet, 1983, 67: 87～9
  • 8[6]Ford J, Malpica J M, Halford N G. The nucleotide sequence of a HMW-GS gene located on chromosome 1A of wheat (Triticum aestivum). Nucleic Acids Res, 1985, 13:6817～6832
  • 9[7]Galili G, Feldman M. Intergenomic suppression of endosperm protein genes in common wheat. Can J Genet Catal, 1984, 26: 651～656
  • 10[8]Lawrence G J, MacRitch F, Wrigly C W. Dough and baking quality of wheat lines deficient in glutenin subunits controlled by the Glu-A1, Glu-B1 and Glu-D1 loci.J Cereal Sci, 1988, 7: 109～112

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华北农学报
2005年 第2期

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