人胎肾小球系膜细胞产生与表达白介素－6

The production and expression of IL-6 infetal mesangial cell (MC)

采用生物学方法与分子杂交技术观察了人胎肾小球系膜细胞产生与表达白介素－６。结果显示系膜细胞上清液中白介素－６活性明显高于对照组（Ｐ＜０．０１），Ｎｏｒｔｈｅｒｎｂｌｏｔ信号扫描分析结果为１０μｇ系胰细胞总ＲＮＡ中白介素－６ｍＲＮＡ是２２．９３；３０μｇ是８４．４４，提示人胎系膜细胞可自然产生白介素－６，并表达白介素－６ｍＲＮＡ。

e have studied the bioactivity and ex-peression of the IL-6 in the fetal glomeruliMC by using biological rnethod and North-ern blot technique. We found that the activi-ty of IL-6 in MC were significantly higherthan in the control (P< 0. 01). The band of10 μg RNA of MC was 22. 93. The band of30 μg RNA of MC was 84. 44. The resultsdemonstrated that the MC could autocrineIL-6 and could transcribe it's rnRNA underunstimu lat ion .