摘要
[目的]观察悦肝胶囊对肝纤维化大鼠肝组织内胶原合成的影响,探讨悦肝胶囊的抗肝纤维化作用机制.[方法]采用二甲基亚硝胺腹腔注射方法制备大鼠肝纤维化模型,分别给予大、小剂量悦肝胶囊.采用生物化学方法测定肝组织内羟脯氨酸含量,采用天狼猩红染色和HE染色法观察肝组织内胶原沉积情况及病理学变化.[结果]口服给予悦肝胶囊明显降低肝纤维化大鼠肝组织匀浆内的羟脯氨酸含量,改善肝组织病理变化.[结论]悦肝胶囊可抑制肝纤维化大鼠肝组织内胶原蛋白的合成,延缓肝纤维化进程.
OBJECTIVETo observe the inhibitory effects and mechanism of the Yuegan capsule on the (synthesis) of collagen in hepatic fibrosis of rats.METHODSThe hepatic fibrosis models in rats were induced by injecting to abdominal cavity with dimethylnitrosamine. The Yuegan capsule was administrated at a large and small dose, respectively. The content of hydroxyproline in liver homogenate was detected. The pathological changes and the deposition of collagen in liver were observed by HE staining and Sirius red staining.RESULTSThe Yuegan capsule significantly reduced the content of hydroxyproline in liver homogenate with improvement of liver histology.CONCLUSIONThe Yuegan capsule could inhibit the synthesis of collagen and delay the process of hepatic fibrosis.
出处
《延边大学医学学报》
CAS
2005年第2期92-96,共5页
Journal of Medical Science Yanbian University
关键词
肝硬变
植物
药用
胶原
大鼠
liver cirrhosis
plants, medicinal
collagen
rats