酶法合成Aspartame的研究

Study on Enzymatic Synthesis of Aspartame

酶法合成Ａｓｐａｒｔａｍｅ的研究许激扬，吴梧桐，姚文兵，金生浩（中国药科大学生化教研室南京２１０００９）关键词酶法合成；化学－酶法；甜味二肽；嗜热菌蛋白酶；木瓜蛋白酶Ａｓｐａｒｔａｍｅ，是由Ｌ－天冬氨酸与Ｌ－苯丙氨酸甲酯组成的二肽甜味剂［１］。近年来...

he thermolysin-catalyzed condensation reaction of N -protected aspartic acid with phenylalaninemethyi ester and papin-catalyzed synthesis of aspartame precursor in biphasic system were. studied.As-partame wJas manufactured by coverting FPM and NH3 donor with E. coli cpu 8901.The enzymaticsynthesis of aspartame and its actual value in ind ustry were discussed.