摘要
背景与目的已有的研究证明癌症的发生需要多种癌基因的协同作用,Bcl2、p27及血管内皮生长因子(VEGF)在肺癌的发生发展中通过不同的途径各自起着不同的作用。本研究拟探讨p27、Bcl2及VEGF基因在肺癌中的表达及其之间的相关性。方法采用免疫组化SP法检测85例肺癌组织、21例肺部良性病变组织中的P27、Bcl2及VEGF的表达水平。结果肺癌组织中VEGF与Bcl2蛋白的阳性表达率分别为65.88%与61.18%,显著高于肺良性病变组织(14.29%与19.05%)(P<0.05)。肺癌组织中P27阳性表达率为37.65%,显著低于肺良性病变组织(80.95%)(P<0.05)。VEGF的表达与肺癌的细胞分化程度、PTNM分期和淋巴结转移密切相关,Bcl2的表达与病理类型、PTNM分期和细胞分化程度密切相关,P27的表达与细胞分化程度密切相关。P27与Bcl2、VEGF之间密切相关,但Bcl2与VEGF之间无明显相关性。结论检测肺癌组织中P27、Bcl2、VEGF的基因表达水平有助于肺癌的诊断和评估肺癌患者的恶性程度。
Background and objective The occurrence of cancer needs many kinds of oncogenes and their cooperation, such as Bcl-2, p27, and vascular endothelial growth factor (VEGF) etc. They have different contributions in lung cancer’s occurrence and development by different paths. In this study the expression of P27, Bcl-2, VEGF and their relationship in lung cancer were investigated. Methods The expression of P27, Bcl-2 and VEGF was detected in 85 lung cancer and 21 benign pulmonary tissues by the immunohistochemistry staining. Results Positive rate of VEGF and Bcl-2 protein expression was 65.88% (56/85) and 61.18% (52/85) respectively in lung cancer tissue, which was higher than that in benign pulmonary tissues (14.29% and (19.05%)) (P<0.05). Positive rate of P27 protein expression in lung cancer tissue (37.65%, 32/85) was significantly lower than that in benign pulmonary tissues (80.95%, 17/21) (P<0.05). VEGF expression was closely related to the grade of cell differentiation, P-TNM stage and lymph node status; Bcl-2 expression was closely related to histology of lung cancer, P-TNM stage and grade of cell differentiation; and P27 expression was closely related to grade of cell differentiation. There was correlation between P27 and Bcl-2 and between P27 and VEGF protein, but no correlation between Bcl-2 and VEGF. Conclusion Detection of P27, Bcl-2 and VEGF will be valuable for dignosing lung cancer and evaluating malignancy extent.
出处
《中国肺癌杂志》
CAS
2005年第3期207-210,共4页
Chinese Journal of Lung Cancer
基金
黑龙江省自然科学基金(DO1—47)资助