摘要
目的:探讨七叶皂甙钠对大鼠局灶性脑缺血再灌注损伤后Bcl2和Caspase3蛋白表达及神经细胞凋亡的影响。方法:采用线栓法制作大鼠大脑中动脉缺血再灌注模型,缺血再灌注模型大鼠随机分为模型组、生理盐水组、七叶皂甙钠组。假手术组手术过程同缺血再灌注模型组,但不闭塞大脑中动脉。采用免疫组织化学染色结合显微图像分析检测再灌后不同时相脑组织缺血半暗带区Bcl2和Caspase3蛋白的动态表达。应用原位末端转移酶标记(TUNEL)染色观察脑组织缺血半暗带区内细胞凋亡的情况。结果:七叶皂甙钠组脑缺血半暗带区Bcl2蛋白表达上调,与模型组及生理盐水组相比差异有统计学意义(P<0.05);七叶皂甙钠组脑缺血半暗带区Caspase3蛋白的表达则明显受到抑制,与模型组及生理盐水组相比差异有统计学意义(P<0.05);同时可见七叶皂甙钠组各时相大鼠脑缺血半暗带区神经细胞凋亡数明显低于模型组及生理盐水组(P<0.01),且凋亡高峰时下降明显。结论:七叶皂甙钠可能通过上调Bcl2表达,下调Caspase3的表达,发挥对脑缺血再灌注损伤的保护作用。
Objective To determine the effects of sodiun aescinate on Bcl-2 and Caspase-3 protein expression and neuronal apoptosis after focal cerebral ischemia reperfusion injury in rats. Methods One hundred male Wistar rats were subjected to middle cerebral artery occlusion (MCAO)and reperfusion.The rats were divided randomly into 4 groups: sham-operated group and MCAO and reperfusion model groups which were randomly divided into control group,saline group,and sodium aescinate group.The immunohistochemistry staining and microscope image were used to observe the dynamic Bcl-2 and Caspase-3 protein expression in the ischemic penumbra after the reperfusion. Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) staining was performed for the detection of apoptosis. Results Bcl-2 protein expression in the sodium aescinate group was significantly higher than that of the saline group and control group (P< 0.05). While Caspase-3 protein expression in the sodium aescinate group was then compared with those of the saline group and control group, and showed the difference was significant (P<0.05).Compared with the saline group and control group,the number of apoptotic cells in the sodium aescinate group was significantly reduced (P<0.01). Conclusion Sodium aescinate increases Bcl-2 protein expression and decreases Caspase-3 protein expression,through which it can protect the ischemia brain on reperfusion injury.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2005年第3期261-265,275,共6页
Journal of Central South University ：Medical Science
基金
国家自然科学基金(30370514)
