应用T细胞受体家族特异性引物的PCR方法对T细胞性淋巴瘤的研究

Detection of T一cell receptor γ chain V gene rearrangements in angioiimmunoblastic lymphadenopathy type T-cell lymphoma by PCR and family specific primers

应用ＰＣＲ方法对１２例血管免疫母细胞性淋巴结病样Ｔ细胞性淋巴瘤（ＡＩＬＤ一ＴＣＬ）进行Ｔ细胞受体γ链（ＴＣＲ＿γ）基因重排分析，结果显示：１２例均有单克隆型的ＴＣＲγ基因重排；而正常人周围血淋巴细胞及非典型性淋巴增生性病变则为多克隆型重排。实验证实ＡＩＬＤ一ＴＣＬ为来自Ｔ细胞的单克隆性肿瘤性疾病。用家族特异性ＴＣＲγ引物对石蜡包埋组织行ＤＮＡ扩增是检测Ｔ细胞性的克隆性增生的敏感、方便、快速的方法，优于传统的Ｓｏｕｔｈｅｒｎ印迹杂交方法，有一定的实用意义。

Using PCR method and family specific primers for T-cell receptor γ chain V gene,the T一cellcolonalities of 12 cases of AILD一TCL were analyzed.The results showed monoclonal DNA amplifica-tion patterns of TCR γin all 12 cases(13 specimens),whereas the DNA from normal peripherallymphocytes and 2 samples of atypical lymphoid hyperplasia showed polyclonal rearrangementpatterns. Our results suggest that AILD一TCL is a monoclonal proliferative disorder originating fromT-cell. The PCR method and family specific primers could successfully amplicate the template DNAfrom paraffin sections,so it is a sensitive, simple and rapid method for routine hematopathologicstudies。