短梗霉多糖发酵条件优化研究

Optimal Culture Conditions of Aureobasidium Pullulans

对长期保存的出芽短梗霉菌种经复壮、筛选后得到一株多糖产量相对高、色素水平相对低的菌株,通过改变培养基渗透压和加入表面活性剂等方法,试图改变细胞膜透性,并针对真菌发酵菌丝易结团的问题进行研究.其中,表面活性剂吐温-80可以解决结团问题并可提高多糖产量.通过正交试验确定最适底物配比,使其多糖转化率达到70%,提高了10%～20%,色素含量减少,发酵液颜色在乳白到淡黄之间,无需脱色处理,即可得到白色粗糖产品.

In order to change the permeability of cell membrane and to solve the problem of linked group of fungi mycelium, the method of adjusting osmotic pressure of medium and adding tween-80 is established.The results show that the problem of linked group can be solved by adding tween-80. The optimal proportion of substrate is determined by means of orthogonal test. The utilized strain with relatively high polysaccride yield and low pigment level is obtained after the rejuvenation and riddling of long-preserved aureobasidium pullulans strain. The transformation ratio of polysaccride is increased by 10%~20% to 70% and the pigment content is greatly reduced. The fermenting liquor is milk white or light yellow, and the white primary product can be gained without decoloration.