堆肥中高效降解纤维素林丹复合菌系的构建及功能

Construction and Function of a High-Efficient Complex Microbial System to Degrade Cellulose and Lindane in Compost

以4种高温期的堆肥样品为材料,经2种筛选方法、多代淘汰及其不同系之间优化组合,最后筛选驯化出一组能有效降解纤维素和林丹的复合微生物菌系.该复合系对滤纸、脱脂棉、稻秸粉和锯末等不同纤维素材料均有较强分解能力,相比之下对天然纤维素含量高的碳源(如滤纸、脱脂棉)分解活性更高.两者的CMC糖化活性在第5d都达到40U以上,分解率达到95%以上.该复合菌系能在较大的pH范围内保持高的纤维素林丹分解活性,在pH为7.0、8.0、9.0条件下林丹降解率均较高,达到45%以上,而纤维素分解率也都在90%以上.而且在pH6～9之间的培养条件下,林丹降解与滤纸分解之间有很好的一致性.

A complex microbial system capable of degrading cellulose and lindane with high efficiency was isolated from four compost heaps. It was selected and domesticated through two methods and by combination of different microbial communities. The results show that the complex microbial system can decompose filter paper, absorbent cotton, rice straw powder and sawdust effectively, especially has high degrading activity for the materials with higher native cellulose such as filter paper and absorbent cotton. As for both of them, the CMC saccharification activity is more than 40U and the degradation efficiency is more than 95% on the 5th day of inoculation. The complex microbial system can also keep a higher degrading capability in a wider range of pH. Filter paper and lindane can be degraded effectively by the complex microbial system during pH 7.0～9.0, and the degradation rates are more than 90% and 45% respectively. Under pH 6.0～9.0, there is a good consistency between the degradation of Lindane and the decomposition of filter paper.