肺癌原癌基因c－Ha－ras点突变的研究

Proto-oncogene Point Mutation of c-Ha-ras in Bronchogenic Carcinoma

应用Ｓｏｕｔｈｅｒｎ印迹杂交技术，在１１例肺癌标本中观察到８例发生了ｃ－Ｈａ－ｒａｓ基因第１２位密码子点突变，突变率为７７．８％。上述肺癌病例中８例取到癌旁组织，发现１例发生了该位点突变，２例兼有突变与未突变杂交带。结合临床、病理资料分析结果提示：ｃ－Ｈａ－ｒａｓ基因第１２位密码子，或突变至少在一部分肺癌病例中并非细胞恶变过程中的起始事件，但可能为早期事件；该位点突变在不同病理类型中未见显著性差异，与ＴＮＭ分期、吸烟史非密切相关。

bronchogenic carcinoma and 8 corresponding paracarcinoma specimens were detected for activating point mutation in codon 12 of c-Ha-ras gene by Southern blotting hybridization technique.The c-Ha-ras gene was found to be activated by point mutation in codon12 in 8 of bronchogenic carcinoma and 1 of paracarcinoma tissues.The mutative and unmutative hybrid band was both discovered in 2 of paracarcinoma tissues,Analysis cf clinical and pathological features , we conclude that:(1)The point mutation of c-Ha-ras gene codon 12 is found in 77.8%bronchogenic carcinoma among Chinese;(2)At least in part cases,mutation of c-Ha-ras gene codon 12 is not the“initiation”event ,but may be a early-stage event in the pathogenesis;(3)No apparent association between the c-Ha-ras gene codon 12 mutation and pathological types,disease stages and history of smoking is present.