毫米波促进周围神经损伤轴突再生的实验研究

Experimental study of the effect of millimeter wave irradiation on the axon regeneration after peripheral nerve injury

目的探讨毫米波对周围神经损伤后轴突再生的影响。方法将48只SD雄性大白鼠制作成左侧坐骨神经部分损伤模型,随机分为治疗组和对照组。治疗组在神经损伤24h后,用毫米波辐射损伤部位,每周5次,每次30min,直至取材的前一天。组织学观察毫米波对大鼠坐骨神经部分损伤后轴突再生的影响。结果治疗组在术后2,4,6周有髓纤维横截面积均显著大于对照组。电镜观察,治疗组在术后4周和6周的神经再生数目和成熟度均优于对照组。结论毫米波能够促进周围神经损伤后轴突的再生。

Objective To investigate the effects of the millimeter wave irradiation on the axon regeneration of injured peripheral nerve. Methods The left sciatic nerves of 48 adult Sprague-Dawley rats weighted around 220 g were crushed with forceps to form the experimental animal model of peripheral nerve injury. Then they were equally divided into a treatment group and a control group: the treatment group received millimeter wave irradiation, while the control group received sham millimeter wave irradiation. The treatment was administered 5 times per week, 30 min per time. The mean axon cross sectional area of the regenerated nerve fibers (ACSA) in two groups were measured and calculated at various stages after operation. Ultra-structural observation was performed with regard to the myelin sheath thickness and maturity of the regenerated fibers. Results It was shown that statistically significant difference existed for the ACSA between the two groups. The Schwann′s cell proliferation, myelin recovery of the treatment group were all better in the treatment group than that of the control group after 4 and 6 weeks post-operation. Conclusion The study suggests that the millimeter wave irradiation could promote the regeneration of peripheral nerve.