摘要
本实验采用同位素〔 ̄3H〕-肌醇参入与阴离子交换树脂层析分离的方法,比较了Wistar大鼠与两肾一环肾性高血压大鼠(2K-ICRHR)主动脉与尾动脉磷酸肌醇(包括-磷酸肌醇IP、二磷酸肌醇IP_2和三磷酸肌醇IP_3)的含量,以及α_1-肾上腺素能受体(α_1-受体)激动剂苯肾上腺素(PE)对它们的影响。结果发现:(1)无论是在静息还是在PE激活的状态下,血管平滑肌中的磷酸肌醇均以IP为主,约占总磷酸肌醇的71-82%;(2)尾动脉中IP_2与IP_3的基础含量明显高于主动脉,提示尾动脉中磷脂酶C(PLC)的基础活性高于主动脉;(3)在PE(10 ̄(-5)mol/L)的作用下,各标本中IP含量均明显增高,反映了PLC被激活。IP增加的幅度在尾动脉高于主动脉,而以RHR尾动脉为最大,但各标本中IP_2与IP_3含量未见明显变化。PE作用后,RHR尾动脉中IP含量明显高于Wistar大鼠。这表明尾动脉α_1受体-PLC通路的活性高于主动脉;(4)基础状态下,尾动脉IP在总磷酸肌醇中所占比例低于主动脉,PE激活后IP所占比例明显增高,而主动脉上述比例无明显变化。提示主动脉与尾动脉被PE激活后,磷酸肌醇代谢途径和方?
By means of  ̄3H myo-inositol labelling and ion
exchange chromatography, contents ofinositol phosphates, including
IP, IP_2 and IP_3, were studied in both aorta and tail artery
ofWistar rats and renal hypertensive rats(RHR, two kidney, one clip).
Effects of phenylephrine(PE),an α_1 agonist,on such contents were
also observed. The results were as follows:(1)In bothresting and
PE-activated arteries, IP took the greatest part(71─82%)in total
amount ofinositol phosphates.(2)The basal contents of IP_2 and IP_3
were significantly higher in tail arterythan in aorta, suggesting
that tail artery has higher basal activity of phospholipase C than
aorta.(3)PE(10 ̄(-5)mol/L)treatment significantly increased IP
contents in all of the arteries, reflectingthe activation of PLC by
PE. while no significant change was observed in contents of IP_2
andIP_3 after PE activation. The increment of IP content was more
significant in tail artery than inaorta, with the largest elevation
found in tail artery of RHR. These indicate that the activity ofα_1
receptor-PLC pathway is higher in tail artery than in aorta.(4)Under
basal condition,theproportion of IP in total inositol phosphates was
lower in tail artery as compared with aorta. PEactivation could
increase such proportion in tail artery but not in aorta, suggesting
that the reac-tion pattern of PLC and phosphatase is different
between the tail artery and aorta.
出处
《中国应用生理学杂志》
CAS
CSCD
1995年第1期25-29,共5页
Chinese Journal of Applied Physiology
关键词
磷酸肌醇
平滑肌
血管
肾性高血压
aorta
tail artery
inositol phosphates
phenylephrine
phospholipase C
renal hypertensive
rat