川芎嗪对人胎肾小球系膜细胞增殖的影响及其机理探讨

Effect of Tetramethylhyrazine on Proliferation of Human Fetal Mesangial Cells

在人胎肾小球系膜细胞（ｍｅｓａｎｇｉａｌｃｅｌｌ，ＭＣ）培养体系中加入川芎嗪后能抑制ＭＣ生长数量。川芎嗪０．６ｍｍｏｌ、１．２ｍｍｏｌ加入ＭＣ培养体系６天后，ＭＣ数量（活细胞个数／ｍｌ）分别为：３７５８０±３４７５，２７３５０±３４１８，明显低于对照组７１８５０±５１０８（Ｐ＜０．０５）；ＭＣ￣３Ｈ－ＴｄＲ摄取量（ｃｐｍ）对照组为３５７５±３０６，加入川芎嗪０．６ｍｍｏｌ、１．２ｍｍｏｌ后分别为１０１７±２０１，５８３±２７１（Ｐ＜０．０１）；加入川芎嗪１ｍｍｏｌ于ＭＣ培养体系中，ＭＣ上清液（１：８稀释度）加入ＫＤ＿（８３）细胞体系中，ＫＤ＿（８３）细胞￣３Ｈ－ＴｄＲ摄取量为２１１８±２１５，明显低于阴性对照组３８９０±２９５与阳性对照组４１２８±３５１（Ｐ＜０．０１）。提示川芎嗪抑制ＭＣ增殖的机理是通过降低ＭＣ自分泌ＩＬ－６而致。

Tetramethylhyrazine (TMP) 0. 6 and 1. 2 mmol were added to human fetal mesangial cell (MC)cultures for 6 days, and the amounts of MC (cells/ml) were 37580±3475 and 27350±3418 respectively,significantly lower than that 71850±5108 in control (P<0. 05) .The 3￣H-TdR incorporation by the MC with corresponding TMP were 1017±201 and 583±271, also significantly lower,than that 3575±306 in control (P<0. 01) . After adding the TMP (l mmol) to the cultures, the IL-6 bioactivity were 2118±215 that were markedly lower than those in controls (4128±351,P<0 . 01). It revealed that the TMP inhibited the growth of MC and the mechanism of its inhibition might be due to that TMP could reduce the IL-6.