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新型胆碱酯酶抑制剂对AD大鼠空间记忆及海马结构胆碱能纤维的影响 被引量:16

Effect of novel AChE inhibitor spatial memory and cholinergic fibers in hippocampal formation of AD rats
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摘要 目的探讨新型胆碱酯酶抑制剂(NAI)及水迷宫训练对AD大鼠海马结构胆碱能纤维的影响。方法用36只Wistar♂大鼠制作AD动物模型,随机分成3组:NAI组、石杉碱甲组(Hup组)、单纯损伤组(SO组)。水迷宫训练后,采用组织化学方法测定海马结构胆碱能纤维密度。结果①定位航行实验中,NAI组逃避潜伏期较SO组显著缩短(P<0.01)。②空间探索实验中,NAI组跨越各象限平台相应位置次数占总次数百分率较SO组明显增高(P<0.01)。③组化结果显示:NAI组海马结构胆碱能纤维密度较SO组明显增加(P<0.05)。尽管NAI组胆碱能纤维密度高于Hup组,但无统计学意义。结论经NAI治疗及水迷宫行为训练后的AD大鼠,海马结构内胆碱能纤维密度明显增加,提示NAI及水迷宫训练联合作用可促进AD大鼠海马结构胆碱能纤维重建。 Aim To investigate the effects of a novel AChE inhibitor (NAI) and water maze training on cholinergic fiber in hippocampal formation of AD rats. Methods 36 male Wister rats of 3~5 months were chosed to make AD models and randomly divided into 3 groups: NAI-, huptrzin A (Hup)-treatment group and simple-injury group. All groups received Morris water maze training, then were tested the AChE positive fiber density in the hippocampal formation by histochemical method. Results ①During the place navigation test,the NAI-treatment group showed shorter latency than the simple-injury group (P<0.01). ②On spatial probe test, NAI-treatment group showed increased percentage of swim time of crossing the platform compared with that of simple-injury group (P<0.01). ③The histochemical study revealed a higher cholinergic fiber density in hippocampal formation of NAI-treatment group than that of simple-injury group. There was no stactistic difference between NAI- and Hup-treatment groups.Conclusion The results showed that NAI treatment and water maze training can increase the cholinergic fiber density in the hippocampal formation of AD rats indicating the function of NAI treatment and water maze training on cholinergic fiber plasticity in the hippocampal formation of AD rats.
出处 《中国药理学通报》 CAS CSCD 北大核心 2005年第7期807-810,共4页 Chinese Pharmacological Bulletin
基金 国家自然科学基金资助项目(No39970865)
关键词 胆碱酯酶抑制剂 水迷宫 胆碱能纤维 AD大鼠 海马结构 乙酰胆碱酯酶(AChE) AChE inhibitor Water maze cholinergic fiber AD rat hippocampal formation AChE
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  • 1Wenk GL. Neuropathologic changes in Alzheimers disease[J]. J Clin Psychiatry,2003,64(Suppl 9):7-10.
  • 2Stahl SM,Markowitz JS,Gutterman EM et al. Co-use of donepezil and hypnotics among Alzheimers disease patients living in the community[J]. J Clin Psychiatry,2003,64(4):466-72.
  • 3Giacobini E. Cholinesterases: new roles in brain function and in Alzheimers disease[J]. Neurochem Res,2003,28(3-4):515-22.
  • 4Kalesnykas G, Puolivali J, Sirvio J et al. Cholinergic neurons in the basal forebrain of aged female mice[J]. Brain Res,2004,1022(1-2):148-56.
  • 5钱亦华,胡海涛.建立Alzheimer病动物模型的方法[J].神经解剖学杂志,1999,15(1):99-102. 被引量:15
  • 6Morris R. Developments of a water-maze procedure for studying spatial learning in the rat[J]. J Neurosci Methods,1984,11(1): 47-60.
  • 7陈啸梅,周文郁,彭俊云 et al. 亚铁氰化铜法示乙酰胆碱酯酶[M]. 组织化学手册,北京: 人民卫生出版社,1982:172-4.
  • 8徐阿晶,陈忠,李韧,朱朝阳,魏尔清.胆碱酯酶抑制剂TAK-147对大鼠空间记忆的作用[J].浙江大学学报(医学版),2002,31(2):98-102. 被引量:5
  • 9朴景华,蒲小平,马建,李长龄,宋志宏北京大学药学院天然药物学系,屠鹏飞.类叶升麻苷对东莨菪碱所致记忆获得性障碍的改善作用[J].中国药理学通报,2001,17(6):625-627. 被引量:39
  • 10Boissiere F, Faucheux B, Ruberg M et al. Decreased TrkA gene expression in cholinergic neurons of the striatum and basal forebrain of patients with Alzheimers disease[J]. Exp Neurol,1997,145(1):245-52.

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