血管通对实验性动脉粥样硬化家兔血管壁血小板衍化生长因子A、B及c－myc基因表达的影响

Effect of Xue Guan Tong on PDGF-A,PDGF-B and c-myc Gene Expression of Vessel Wall in Atherosclerotic Rabbits

本实验采用斑点印迹杂交和原位杂交技术，进一步探讨血管通对与血管平滑肌细胞（ＶＳＭＣ）增生相关基因表达的影响。结果表明，高脂组家兔血管壁血小板衍化生长因子（ＰＤＧＦ）－Ａ的ｍＲＮＡ呈高表达，杂交阳性颗粒主要分布在动脉粥样硬化（ＡＳ）斑块边缘区及新生斑块区的ＳＭＣ胞浆内，而血管通组家兔血管壁ＰＤＧＦ－ＡｍＲＮＡ表达水平明显低于高脂组；高脂组血管斑块组织ｃ－ｍｙｃ的ｍＲＮＡ表达水平亦比正常组高，血管通组血管壁ｃ－ｍｙｃｍＲＮＡ表达水平下降。上述结果初步说明血管通可能是通过影响血管壁ＰＤＧＦ－Ａ、ｃ－ｍｙｃ的ｍＲＮＡ表达水平，以抑制ＶＳＭＣ增生，阻止ＡＳ形成。

In this study the effect of Xue Guan Tong (XGT)on platelet-derived growth factor-A(PDGF-A),PDGF-B and c-myc gene expression of vessel wall was explored with dot blot and in situ hybridization.The results shown that the expression of PDGF-A mRNA in atherosclerotic plaque of the model group was higher,PDGF-A gene hybridizations were mainly distributed in smooth muscle cell which located in newly emerging plaque and edge of atherosclerotic plaque.In contrast,level of PDGF-A mRNA in the XGT group was lower than that in the model group,which indicated that XGT could influence the level of PDGF-A mRNA.The c-myc oncogene expression in the model group was significantly enhanced than that in the normal group.However,the expression in XGT group decreased less than that in the model group.From the results shown above, it suggested that one of the effects of XGT on preventing VSMC proliferation was its influence on PDGF-A and c-myc mRNA expression level.