急性甲型肝炎患者粪便排毒期的观察

STUDYONTHE PERIODOFFECALEXCRETIONOF HEPATI TI SAVI RUSI NPATI ENTSWI THACUTE HEPATITIS A

收集急性甲型肝炎（甲肝）患者３０例发病后１～６周粪便，制备悬液作甲型肝炎病毒（ＨＡＶ）培养，收集细胞液，应用斑点杂交检测ＨＡＶＲＮＡ，同时用ＥＬＩＳＡ法检测甲肝抗原。结果发现：（１）发病后第１周粪便ＨＡＶＲＮＡ检出率占７０％；（２）发病后第４周检出５例（１６．７％），第５周尚检出１例；（３）粪便悬液经细胞培养后，第５周尚有１例ＨＡＶＲＮＡ阳性。提示少数甲肝恢复期患者粪便排毒期可达５周。

3 0 cases of HA were stud ied.Feca l specimens were co l lected f rom the first up tO the sixth weeks after onset in the patients’with hepafitis typeA.Supernates of the cellcu ltures wasdetectedforHAVRNAbyDotBlothyrzation,andfor HAV atigen byELISA technique. The results showed that:(1) The positive rate of fecal HAV RNAwas 70%(21/30) in the first week of the illness.(2) Fecal HAV RNA were positivein 5 patients in the fourth week,and in one patient in the fifth week of the illness. (3)In a patient the HAV was still detectable in the supernate of fecal culture in thefifth week,it indicates that the infectivity of stool lasted at least for 5 weeks.Inarare patient,fecal shedding o f HAV coul d continue up to f ive weeks af ter onset.