知母药材高效液相指纹图谱研究

Study on chromatographic fingerprints of rhizoma anemarrhenae

用高效液相色谱法建立了知母药材的指纹图谱分析方法。采用ZorbaxEclipseXDB C18色谱柱(5μm,4.6×250mm,Agilent),流动相为乙腈和25mmol/LKH2PO4缓冲液,梯度洗脱;流速:1.0mL/min;柱温为25℃;检测波长257nm。确定了8批不同产地知母药材甲醇提取部分6个共有峰,各产地药材指纹图谱与对照指纹图谱相似度良好(>0.9),可以用来作定性研究。方法准确可靠,为提高知母药材质量控制标准提供参考。

<Abstrcat>The HPLC Fingerprints of rhizoma anemarrhenae was established,based on chromatograms of 8 batches of samples ,which obtained from different sources. The Zorbax Eclipse XDB-C_(18) column(5 μm, 4.6×250 mm, Agilent)was used as an analytical column with the mobile phase A:acetonitrile and B: 25 mmol/L phosphate buffer with gradient elution in 60 min. The flow rate was 1.0 mL/min and the column temperature was controlled at 25 ℃, and the detection wavelength was set at 257 nm. 6 common peaks were found in the fingerprints of 8 batches of rhizoma anemarrhenae, and good similarities with correlation coefficients higher than 0.9 were found in fingerprints between the herbs from different sources and the standard chromatographic fingerprint, which can be utilized for the identification of rhizoma anemarrhenae. The method is accurate and reproducible, providing a new reference for quality control of rhizoma anemarrhenae.