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食管癌组织中抑癌基因APC、MCC突变的研究 被引量:7

Mutation of Tumor Suppressor Genes APC and MCC in Human Esophageal Cancer
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摘要 应用聚合酶链反应(PCR)扩增与直接测序方法,分析抑癌基因APC、MCC在食管癌中的变化,应用PCR扩增,发现1/10的食管癌组织有APC基因第11外显子缺失,1/10的食管癌组织有MCC基因第12外显子缺失,并发现1例食管癌旁癌组织有MCC基因第12外显子缺失。PCR直接测序发现:2110的食管癌标本有APC基因第11外显子突变,2/7的食管癌组织有MCC基因第12外显子突变。以上研究证实食管癌组织中存在APC、MCC基因点突变,为食管癌癌变研究提供了新的线索。有关APC、MCC基因丢失与突变的机制需要进一步研究。 Abstract The mutation and deletion of APC ,MCC genes in human esophageal cancer were analyzed byPCR amplification and direct sequencing assay,In PCR amplification analysis,one of 10 cases ofesophageal cancer was found to have APC gene deletion in exon 11;one of 10 cases of EC was foundto have MCC gene deletion in exon 12; one case of EC was found to have MCC gene deletion in exon12.One of adjacent non-tumor tissue was also found to have deletion at exon 12 of MCC.In PCR di-rect sequencing analysis,two of 10 cases of EC were found to contain APC gene mutation in exon 11,two of 7 cases of EC were found to contain MCC genes mutation in exon 12.The results confirmedthat mutation of APC and MCC genes exists in human esophageal cancer.It gives new clues to the un-derstanding of carcinogenesis of human esophageal cancer.The mechanism of mutation or deletion ofAPC and MCC genes in EC needs further study.
出处 《中华肿瘤杂志》 CAS CSCD 北大核心 1995年第1期9-12,共4页 Chinese Journal of Oncology
基金 国家"八 五"科技攻关资助课题
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  • 1李华川,中华肿瘤杂志,1994年,16卷,172页
  • 2李华川,中华肿瘤杂志,1993年,15卷,412页
  • 3李华川,中华肿瘤杂志,1990年,12卷,161页

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