摘要
应用聚合酶链反应(PCR)扩增与直接测序方法,分析抑癌基因APC、MCC在食管癌中的变化,应用PCR扩增,发现1/10的食管癌组织有APC基因第11外显子缺失,1/10的食管癌组织有MCC基因第12外显子缺失,并发现1例食管癌旁癌组织有MCC基因第12外显子缺失。PCR直接测序发现:2110的食管癌标本有APC基因第11外显子突变,2/7的食管癌组织有MCC基因第12外显子突变。以上研究证实食管癌组织中存在APC、MCC基因点突变,为食管癌癌变研究提供了新的线索。有关APC、MCC基因丢失与突变的机制需要进一步研究。
Abstract The mutation and
deletion of APC ,MCC genes in human esophageal cancer were analyzed
byPCR amplification and direct sequencing assay,In PCR amplification
analysis,one of 10 cases ofesophageal cancer was found to have APC
gene deletion in exon 11;one of 10 cases of EC was foundto have MCC
gene deletion in exon 12; one case of EC was found to have MCC gene
deletion in exon12.One of adjacent non-tumor tissue was also found to
have deletion at exon 12 of MCC.In PCR di-rect sequencing
analysis,two of 10 cases of EC were found to contain APC gene
mutation in exon 11,two of 7 cases of EC were found to contain MCC
genes mutation in exon 12.The results confirmedthat mutation of APC
and MCC genes exists in human esophageal cancer.It gives new clues to
the un-derstanding of carcinogenesis of human esophageal cancer.The
mechanism of mutation or deletion ofAPC and MCC genes in EC needs
further study.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1995年第1期9-12,共4页
Chinese Journal of Oncology
基金
国家"八
五"科技攻关资助课题