林县食管癌中p53基因突变与p53高表达相关性的研究

CORRELATION BETWEEN p53NUCLEAR PROTEIN ACCUMULATION AND MUTATIONS OF THE p53 GENE IN HUMAN ESOPHAGEAL CANCER FROM LINXIAN

用免疫组织化学染色和ＰＣＲ直接测序法分别检测了食管癌高发区林县食管癌组织中Ｐ５３蛋白表达水平和基因突变以及二者的相关性。在３３例食管癌标本中，２３例发现有Ｐ５３的高表达，１０例未发现可检测到的Ｐ５３蛋白，其阳性率为６９．７％。Ｐ５３基因第５→８外显子出现点突变和缺失者１２例，其频率为３６％。在１２例ｐ５３基因突变的病例中，９例均有Ｐ５３蛋白的高度表达，提示ｐ５３基因突变与Ｐ５３蛋白的表达密切相关，并是造成Ｐ５３高表达的重要原因之一。Ｐ５３突变和Ｐ５３蛋白表达在食管癌组织中发生的高频率说明：Ｐ５３的改变是食管癌癌变过程中重要的生物学事件之一。

AbstractThirty-three esophageal tumors were analyzed by immnohistochemistry for p53 nuclear pro-tein accumulation,and the results were compared to p53 gene mutations by PCR-direct sequenceanalysis.A highly significant correlation between the presence of p53 mutations and p53 nuclearprotein accumulation was found.Of 33 tumors ,23(69.7%)that demonstrated p53 protein x-pression 12(36%)had p53 mutations. Of 12 tumors with p53 mutations,9 tumors showed p53 in-tensive nuclear reactivity. The results reported here are consistent with the idea that p53 muta-tions may be an important biological event in esophageal cancer progression and p53 expressionwas correlated with p53 gene mutation.