丹参和钙通道拮抗剂抗LDL氧化作用的对比研究

The comparison between radix salviae miltiorrhizae(RSM )and nisoldipine nifedipine on the effect of antiperoxidation of low density lipoprotein

提取健康成人血浆中的低密度脂蛋白（ＬＤＬ），与丹参和硝苯吡啶、尼索地平及Ｃｕ透析，分别于透析后２４、４８及７２小时从各组ＬＤＬ中提取脂类进行薄层层析（ＴＬＣ）。结果显示：①ｃｕ可使ＬＤＬ氧化成ｏｘＬＤＬ，ＴＬＣ示ｘ带、游离脂肪酸（ＦＦＡ）、磷脂（ＰＬ）增加，而胆固醇酯（ＣＥ）及甘油三酯（ＴＧ）减少；②钙通道拮抗剂＋Ｃｕ透析与单纯Ｃｕ透析结果相似；③丹参＋Ｃｕ透析的ｘ带显著低于Ｃｕ透析组及钙通道拮抗剂组，且脂质成分与透析前相似。结果表明，Ｘ带可作为脂质过氧化的一个重要指标，丹参具有拮抗ＬＤＬ氧化的作用，这可能是其防治心脑血管病的重要机理之一。

The oxidized LDL generated by dialysing LDL from healthy persons with CuCl2 solution was used as a model to investigate the antioxidate property of RSM and nisoldipine nifeldipine(Ni). The results showed that the spot X was clearly found on the thin layer chromatography(TLC)after dialysis of LDL with CuCl2 solution or with nisoldipin nifedipine and CuCl2. However, the spot X was not found in RSM group.The analysis of lipid constituents in LDL by scanning showed that the percentage of spot X in RSM group was significantly lower than those in Cu group and Ni groups. The results indicated that the spot X may be an important indicator for lipoprotein peroxidizability and RSM plays a potential role in antioxidation of LDL Iipids.