摘要
目的:观察茴三硫对大鼠急性酒精性肝损伤动物模型的防治作用。方法:健康雄性Wistar大鼠60只,随机分为6组。A组(空白对照组)灌胃生理氯化钠溶液20mL·kg-1·d-1;B组(模型组)灌胃等剂量白酒;C,D,E组(茴三硫小,中,大剂量组)在B组基础上分别预先灌胃茴三硫5,10,20mg·kg-1·d-1,F组(阳性药对照组)在B组基础上预先灌胃硫普罗宁100mg·kg-1·d-1;实验共10d。实验d6和d11取各组大鼠血液,检测谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酯甘油(TG)、胆固醇(CHO)、谷胱甘肽过氧化物酶(GSH-Px)和丙二醛(MDA);实验结束时处死动物,取肝组织,进行HE和Masson染色,光镜检查。结果:在d6和d11时B组大鼠血清ALT,AST,TG和MDA较A组显著升高,GSH-Px显著降低;C,D,E,F组较B组均有所改善。病理检查发现C,D,E,F组大鼠肝脏脂变、炎症坏死均有所减轻,前者改善明显。结论:茴三硫对大鼠急性酒精性肝损伤有明显的防治作用。
Objective: To evaluate the protective and therapeutic effects of anetholtrithione on acute alcoholically hepatic injury in rats. Methods:Sixty male Wistar rats were randomly assigned to one of the six groups: Group A (placebo group) was orally given with saline of 20 mL·kg^-1·d^-1; Group B was drunk with 56% alcohol of 20 mL·kg^-1·d^-1; Group C, D and E was orally supplemented with anethohrithione of 5, 10 and 20 mL·kg^-1·d^-1 and subsequently drunk with alcohol, respectively; Group F (positive control) was fed with tiopronin of 100 mL·kg^-1·d^-1 and subsequently drunk with alcohol. All rats were treated for 10 days. At the 6th and 11 th day of the treatment, the rat bloods were collected to measure alanine amino transaminase (ALT), aspartate amino transaminase ( AST), triglycerides ( TG), cholesterol ( CHO ), glutathione peroxidase (GSH-Px), malondialdehyde (MDA). At the end of the treatment, rats were euthanized to conduct the histopathological examination. Results: Compared with other groups, Group B showed severe hepatic injury with abnormally higher levels of ALT, AST, TG and MDA, and lower GSH-Px, as well as hepatocyte fatty degeneration, cellular necrosis and inflammatory infiltration. Rats fed with anetholtfithione and tiopronin experienced mild abnormalities in the blood tests and hepatic injury. Conclusion:Anethohfithione may prevent rats from acute alcohol-induced hepatic injury.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2005年第7期852-855,共4页
Chinese Journal of New Drugs
关键词
茴三硫
急性酒精性肝损伤
肝功能
血脂
anetholtrithione
acute alcohol-induced hepatic injury
hepatic function
blood fat