抑郁症易感性与5-羟色胺转运体基因的多态性

Susceptibility of depression and the polymorphisms of serotonin transporter gene

目的:探讨5-羟色胺转运体启动子区域基因多态性和抑郁症之间是否存在关联性。方法:运用聚合酶链反应和限制性片断长度多态性技术,检测抑郁症患者(患者组,n=60)和正常人(对照组,n=65)的5-羟色胺转运蛋白启动子区多态性的分布频率。结果:按意向处理分析,纳入结果分析患者组为60例,对照组为65人。①5-羟色胺转运蛋白启动子区基因型和等位基因频率比较:患者组和对照组5-羟色胺转运蛋白启动子区的S/S基因型频率分别为43.3%和20.0%,S等位基因频率分别为60.8%和43.8%,差异均有显著性(χ2=7.591,7.214;P<0.05)。②5-羟色胺转运蛋白启动子区基因多态性:所有被检测者共出现3种基因型:纯合子L/L(419bp,419bp)、杂合子S/L(375bp,419bp),S/S(375bp,375bp)。结论:抑郁症患者比正常人有较高频率的S/S基因型和S等位基因,5-羟色胺转运蛋白启动子区的S等位基因可能是抑郁症的易感基因之一。

AIM： To Investigate whether there is association between the polymorphism of serotonin transporter gene-linked polymorphie region and depression.METHODS： The distributive frequencies of serotonin transport protein gene-linked polymorphic region of 60 depressive patients （patient group） and 65 normal people （control group） were detected with the polymerase chain reaction and restriction fraction length polymorphism technique.RESULTS： According to intention-to-treat analysis, 60 eases in the patient group and 65 cases in the control group were involved in the analysis of results. ① The frequencies of short/short （S/S）genotype in the serotonin transport protein gene-linked polymorphic region in the patient group and control group were 43.3% and 20.0% respectively, and the frequencies of S allele were 60.8% and 43.8% respectively, there were significant differences between the two groups （x^2=7.591, 7.214; P 〈 0.05）. ② Three genotypes were detected in all the subjects： homozygote of L/L （419 bp, 419 bp）, heterozygotes of S/L （375bp, 419 bp） and S/S （375 bp, 375 bp）.CONCLUSION： Patients with depression have higher frequencies of S/S genotype and S allele than normal people; S allele in serotonin transport protein gene-linked polymorphic region may be one of the predisposing genes of depression.