温肾健脾方对大鼠慢性创面愈合的影响

Effects of Wenshen Jianpi Recipe on chronic wound healing in rats

目的:探讨温肾健脾中药对慢性创面愈合的影响及可能作用机制。方法:采用大鼠背部开放性创面模型,用肌注氢化可的松的方法造成慢性难愈性创面,以辛葡康为阳性对照药,应用免疫组化、流式细胞术等方法,观察创面愈合时间、新生上皮宽度、肉芽组织形态学变化、肉芽组织细胞周期、表皮生长因子(epidermal growth factor, EGF)、转化生长因子β1(transforming growth factor β1, TGF β1)及纤维连接蛋白(fibronectin, FN)等指标。结果:温肾健脾方治疗组与辛葡康对照组平均创面愈合时间分别为(17.0±1.9)和(18.8±1.9) d,较模型组及空白组明显缩短(P<0.05);治疗14 d后,治疗组及对照组新生上皮宽度分别为(3.73±0.19)和(3.21±0.15)mm,较模型组及空白组明显增宽(P<0.05);治疗组新生毛细血管丰富,成纤维细胞数量多,而对照组、模型组及空白组新生毛细血管及成纤维细胞数量均较治疗组明显减少(P <0.05);治疗组S期细胞比例较对照组、模型组及空白组明显提高(P<0.05);治疗组及对照组与模型组及空白组比较,EGF、TGF -β1、FN蛋白表达均增强(P<0.05)。结论:温肾健脾方有促进大鼠慢性难愈创面修复的作用,其机制与调节细胞周期,上调EGF、TGF -β1细胞因子蛋白表达水平,促进间质FN的表达有关。

Objective： To study the effects of Wenshen JJanpJ Recipe （WSJPR, a traditional Chinese medicine for warming kidney and invigorating spleen） on chronic wound healing and the mechanism.Methods. Ninety-six SD rats were randomly divided into 4 groups, with 24 rats in each group, and back wound was made in the rats. For rats in 3 of the 4 groups, hydrocortisone injection was administered to induce chronic wound. Rats in 2 of the 3 groups were treated with WSJPR and Xinpukang Granules （XPKG）respectively, and the rats in the other group were untreated. The rats in the fourth group were taken as control. The wound healing time and the width of new epidermis were observed, and the histomorphological changes and cell cycle of the granulation tissue, and the protein expressions of epidermal growth factor（EGF）, transforming growth factor-β1 （TGF-β1） and fibronectin （FN） in the granulation tissue were tested with immunohistochemical technique and flow cytometry. Results. The wound healing time of the WSJPR-treated and XPKG-treated groups was （17.0 ± 1.9） and （18.8 ± 1.9） d respectively, much shorter than that of the untreated and control groups （P＜0.05）. On the 14th experiment day, the width of new epidermis of the WSJPR-treated and XPKG-treated groups was （3. 73 ± 0. 19） and （3.21 ± 0. 15） mm respectively, much wider than that of the untreated and control groups （P＜0.05）. The numbers of angiogenesis, fibroblasts and cells in the S phase in WSJPR-treated and XPKG-treated groups were much higher than those in the untreated and control groups （P＜0.05）. Compared with the untreated and control groups, the protein expressions of EGF, TGF-β1 and FN in WSJPR-treated and XPKG-treated groups were higher （P＜ 0.05）. Conclusion：WSJPR can enhance the wound healing. It was likely through accelerating the cell proliferation and upregulating the expressions of EGF, TGF-β1 and FN.