硅胶柱层析纯化青蒿素

The process of artemisinin purification by silica gel column chromatography

目的确定硅胶柱层析对超临界CO2萃取所得青蒿素粗品纯化的工艺条件。方法采用薄层层析-硅胶柱层析法考察不同展开剂的层析效果,最优洗脱剂为正己烷-乙醚(80∶20);以青蒿素回收率和平均含量为评价指标,考察了流速、进样量与层析柱再生条件对层析分离的影响。结果硅胶柱层析纯化青蒿素的最佳操作条件为洗脱剂空塔流速0.5cm.min-1,进样量18.9mg.ml-1柱体积,甲醇再生2倍床层体积。青蒿素含量可由原来的15%提高到70%以上,回收率达90%。经过重结晶精制,产品中青蒿素含量超过99.5%。结论硅胶柱层析纯化青蒿素所得产品符合质量要求。

OBJECTIVE To study the purification of artemisinin by silica gel column chromatography conducted after the supercritical fluid extraction. METHODS Thin layer chromatography - silica gel column chromatography was used to analyze the effect of different mobilephases and confirm the best mobile phase （n - hexane：ether = 80：20） （by volume）. And the effects of flow rate, sample load ,and the regenerated condition were. further researched on the purity and the recovery of artemisinin. RESULTS The optimal operation conditions wereflow rate 0.5 cm＇min-I, sample load 18.9 mg·ml^-1. The silica gel column might be regenerated by 2 bed volumes of methanol. The purity of artemisinin could be improved from 15% to more than 70% after purification, and the recovery was 90%. The purity of artemisinin could be further reached 99.5% by recrystallization. CONCLUSION After the process of silica gel column chromatography, the artemisinin can meet the demand of the market.