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黄芩的抗菌活性及HPLC分析 被引量:17

Antimicrobial Activity of Scutellaria Baicalensis Georgi and HPLC Analysis
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摘要 将黄芩用V(乙醇)∶V(水)=70∶30进行提取,提取物(SC)按极性大小分成四个部位:正己烷(SC1),乙酸乙酯(SC2),正丁醇(SC3),水部位(SC4),用打孔法测定每个部位的抑菌圈,用试管稀释法测定SC2,SC3和SC4对大肠杆菌和枯草杆菌的最低抑菌质量浓度(M IC)分别为2,>2,>2 g/mL;1,2,>2 mg/mL。HPLC对总提物及各个部位的主要成分进行了定性分析,结果表明,SC的主要成分为黄芩素、汉黄芩素和黄芩苷;SC2的主要成分为黄芩素和汉黄芩素;SC3主要成分为黄芩素和黄芩苷;SC4的主要成分为黄芩苷。 A crude extract of Scutellaria baicalensis Georgi (huang-qin) (SC) was partitioned with n-hexane ( SC1 ), ethyl acetate ( SC2 ) , n-butanol ( SC3 ) and water ( SC4 ). The antimicrobial activities of the crude extract and four sub-fractions were reported. The antimicrobial assays included well difussion assay and growth inhibition by broth dilution tests. The minimal inhibitory concentration (MIC) of SC2, SC3 and SC4 for Eseheriehia Coli and Bacillus Subtilis were 2, 〉2, 〉2 mg/mL and 1,2, 〉2 mg/mLrespectively. HPLC analysis showed that the major substances of crude extract were baicalin, baicalein and wogonin; the major substances of SC2 were baiealein and wogonin; the major substances of SC3 were baiealin and baiealein;and the major substance of SC4 was baiealin.
出处 《精细化工》 EI CAS CSCD 北大核心 2005年第8期589-591,共3页 Fine Chemicals
关键词 黄芩 抗菌活性 HPLC Scutellaria baicalensis Georgi antimicrobial activity HPLC
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  • 1周邦靖,徐有穗.160种中药对小肠结肠炎耶氏菌的抗菌作用[J].成都中医学院学报,1993,16(4):36-37. 被引量:18
  • 2吕小迅,周玉珍,方丹云,吕华冲.黄芩黄精等四种中药抗真菌实验研究[J].广东药学院学报,1995,11(1):18-19. 被引量:20
  • 3吕小迅,周玉珍,方丹云,吕华冲.黄芩黄精联合应用的抗真菌实验研究[J].中国皮肤性病学杂志,1996,10(2):80-80. 被引量:10
  • 4李仲兴 郑家齐.诊断细菌学[M].香港:黄河文化出版社,1992.295-297.
  • 5曹仁烈 孙在源 等.中药水浸剂在试管内抗皮肤真菌的观察[J].中华皮肤科杂志,1957,(4):286-290.
  • 6刘国声.中药抗生力研究(第一报)[J].中华新医学报,1950,1(2):95-97.
  • 7163医院药局.中药汤剂煎煮方法研究[J].中草药通讯,1976,(1):16-18.
  • 8孙迅.黄连黄芩等煎液抗皮癣真菌作用的研究[J].中华医学杂志,1955,41(6):536-536.
  • 9陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1991.259-262.
  • 10李楚銮 林书 王煜华.44种中药草药及其复方对各种痢疾的抗菌作用观察[J].福建中医药,1960,5(7):38-41.

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