补阳还五汤及其主要有效部位对小鼠动脉血栓形成的影响

Effect of Buyang Huanwu Decoetion and its Basis Fractions on Arterial Thrombosis in Mice

目的研究补阳还五汤及其生物碱、苷两类有效部位对小鼠角叉菜胶所致动脉血栓形成的影响,探讨该方抗动脉血栓形成的物质基础。方法小鼠背部皮下注射1％角叉菜胶(50mg／kg),同时给予药物,于24h后观察小鼠尾动脉血栓形成长度。结果补阳还五汤原方腹腔注射生药2.5、5、10g／kg,均可使小鼠24h后尾动脉血栓形成长度显著性缩短(P＜0.01)；由该方提取的有效部位生物碱,在腹腔注射0.075、0.15 g／kg时,对小鼠尾动脉血栓形成无明显影响(P＞0.05)。在剂量为0.3g/kg时,可使尾动脉血栓长度缩短(P＜0.05)。苷在剂量为0.125、0.25g／kg时也对尾动脉血栓长度无明显影响(P＞0.05),剂量为0.5g／kg时,也可使血栓长度缩短(P＜0.05)。对照药物低相对分子质量肝素LMWH(750u／kg)和噻氯匹啶(0.05g／kg)均可使尾动脉血栓形成长度缩短(P＜0.05)。结论抗凝血药(LMWH)和抗血小板药噻氯匹啶可抑制角叉胶所致的尾动脉血栓形成,表明该模型与凝血功能亢进和血小板活化有关。补阳还五汤及其主要有效部位生物碱和苷可抑制该模型血栓形成,提示该方的作用可能与抑制凝血亢进和血小板活化有关。该方中生物碱和苷可能为其抗血栓的主要有效物质基础。

Objective To investigate offect of buyang huanwu decoction（ BHD） and two kinds of fractions extracted from BHD on tail artery thrombosis induced by carrageenan in mice. To explore active components of BHD antagonizing arterial thrombosis , Methods Mice were administered subcutaneously with 1% carrageenan and drugs simultaneously. Lengths of tail artery thrombosis were observed after 24 h in mice. Resuits Lengths of tail artery thrombosis at doses of 2.5,5, and 10 g crude medicine/kg ip in BHD groups shortened significandy after 24 h （P 〈0.01 ） ,Lengths of thrombosis at doses of 0.075 and 0.15g/kg ip in alkaloid extracted from BHD groups had no marked changes（P 〈0.05） ,but thrombosis lengths at a dose of 0.3 g/kg ip in alkaloid group shortened （P 〉0.05） ,Glycocide extracted from BHD at doses 0. 125 and 0.25 g/ kg ip did not influences thrombosis lengths ,but thrombosis lengths at a dose of 0.5g/kg ip in glycoside group shortened （ P 〈 0.05 ）. Thrombosis lengths in low molecular weight heparin （LMWH） group （ 750 u/kg） and in ticlopidine group （0.05 g/kg） shortended （ P 〈 0.05 ）. Conclusion Anticoagulant LMWH and antiplatelet ticlopidine could inhibit tail artery thrombosis induced by carrageenan in mice. This suggested that thrombosis model relates to hyperfunction of blood coagulation adn plaelet activation. BHD and its basis fractions could inhibit thrombosis in the model. This means that effect of BHD relates to inhibiting hyperfunction of blood coagulation and platelet activation. Alkaloid and glycoside are possible basic active components of BHD antagonizing thrombosis.