马铃薯试管块茎形成机制的研究──BA对试管块茎形成与膨大的影响

RESEARCH ON THE FORMING MECHANISM OF POTATO MICROTUBERS──EFFECTS OF BA ON THE FORMATION AND GROWTH OF MICROTUBERS

试验以“米拉”脱毒苗为基础材料，研究了在不同光照和蔗糖浓度条件下，不同浓度ＢＡ以及不同时间加入ＢＡ对试管块茎形成与生长的影响．试验结果表明，ＢＡ与光照条件、蔗糖浓度之间在影响块茎形成和生长过程中不存在显著的互作关系，但三者均为块茎形成和膨大的必要条件．短日照有利于匍匐茎的发生，ＢＡ有利于匍匐茎顶端的膨大，蔗糖浓度与块茎大小和数量有密切关系．本文涉及的试验中，每天光照８小时，蔗糖浓度为８％，使用２ｐｐｍＢＡ在转入短日照８～１０天后加入对块茎的形成与膨大效果最好，最后有效单株块茎数达２．２４和２．２６个。

Virus-free plantlets of potato cv. Mira produced in vitro were used for the experiments.The effects of BA with dirferent concentration and supplemental time on the formation andgrowth of microtubers were investigated. The results showed that there was no obvious mutual correlation among BA, sugar concentration and light condition in terms of the effects on in itiation and growth of microtubers, but they were individually essential for the tuber forma-tion and tuber growth. Short day was in favour of stolon initiation while BA accelerated bulking of the stolon tips and sugar concentration was closely relevant to number and size ofmicrotubers. The best results were observed when the cultures were transfered to 8h with themedium supplementcd with 2ppm BA after 8 and 10 days in short day and 8% sucrose. Themean number of microtubers per plantlet was 2.24 and 2.26, rcspcctivcly.