期刊文献+

大鼠酪氨酸羟化酶基因在肌母细胞中稳定表达 被引量:1

Stable Expression of Rat Tyrosine Hydroxylase Gene in Myoblast Cell Line
下载PDF
导出
摘要 用电穿孔法将大鼠酪氨酸羟化酶(Tyrosinehydroxylase,TH)基因转染大鼠L-6TG成肌细胞株,经PCR检测、免疫组织化学和荧光组织化学检测证明,TH基因能在细胞内稳定整合和表达,并在辅因子存在时将酪氨酸转化为多巴.移植于大鼠纹状体后可成活并表达TH。 Tyrosine hydroxylase (TH) may serve as target gene in gene therapy for Parkinson disease (PD). By electroporation,rat TH cDNA was introduced into the rat myoblast cell line L-6TG. Stably integrated cell line was cloned and expressionof TH was detected in the transformed cells by immunohistochemical staining. When supplying cofactor biopterin in the medium,TH expressed in myoblast converted tyrosine to L-dopa. After transplanted into rat striatum for 1month,the TH-myoblasts could still survive and express TH in vivo.
出处 《生物化学杂志》 CSCD 1995年第3期359-362,共4页
基金 "863"-102-165和国家自然科学基金
关键词 酷氨酸羟化酶 基因 基因表达 震颤性麻痹 Tyrosine hydroxylase Myoblast Gene expression Intracerebral transplantation
  • 相关文献

参考文献1

二级参考文献1

  • 1黄淑帧,上海医学,1988年,11卷,559页

共引文献14

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部