正交设计在诱导植物体细胞胚胎发生中的应用

APPLICATION OF ORTHOGONAL EXPERIMENT ON INDUCTION OF PLANT SOMATIC EMBRYOGENESIS

利用正交试验方法研究了培养基中主要成分，ＮＨ，ＮＯ，Ｃａ￣（２＋），Ｍｇ￣（２＋）对党参体细胞胚胎发生的影响。发现３０ｍｍｏｌ／ＬＮＨ＋３０ｍｍｏｌ／ＬＮＯ组合最有利于体细胞胚胎发生，其它依次为３０ｍｍｏｌ／ＬＮＨ、３０ｍｍｏｌ／ＬＮＯ、３０ｍｍｏｌ／Ｌ ＮＨ与０．５ｍｍｏｌ／ＬＣａ￣（２＋）、３０ｍｍｏｌ／ＬＮＯ与７．０ｍｍｏｌ／ＬＣａ￣（２＋）、３．０ｍｍｏｌ／ＬＣａ￣（２＋）、１．５ｍｍｏｌ／ＬＭｇ￣（２＋）。通过分析得出优选培养基成分为３０ｍｍｏｌ／ＬＮＨ、３０ｍｍｏｌ／ＬＮＯ、１．０ｍｍｏｌ／ＬＣａ￣（２＋）和１．５ｍｍｏｌ／ＬＭｇ￣（２＋），其它成分均同ＭＳ培养基成分。用此培养基，党参体细胞胚胎发生比ＭＳ培养基提高２．５倍。

The main composition of culture medium such as NH,NO,Ca￣(2+),Mg￣(2+) were tested fortheir effects on Dangshen [Condonopsis pilosula (Franch)Nannf.] somatic embryogenesis fre-quency by means of Orthogonal experiment. The result showed that the interaction of 30 mmol/L NH and 30 mmol/L NO played an important role in induction of embryogenesis. The otherin order: 30 mmol/L NH, 30 mmol/L NO_3, 30 mmol/L NH and 0. 5 mmol/L Ca￣(2+),1.5mmol/L Mg￣(2+), Analysing the result and getting this medium compositions are as follows : 30mmol/L NH.30 mmol/L NO_3 ,1. 0 mmol/L Ca￣(2+),1. 5 mmol/L Mg￣(2+),others are the same asMS medium.This medium increase somatic embryo yield 2. 5 fold.