摘要
采用６％羟乙基淀粉（ＨＥＳ）和５％二甲基亚砜（ＤＭＳＯ）为冷冻保护剂，不用程控降温装置和液氨，直接于一８０℃冰箱中冻存外周血造血干细胞（ＰＢＳＣ）。于冻存前和冻存后１周、１个月、２个月和３个月分别取样分析。冻存３个月后，外周血单核细胞（ＭＮＣ）回收率为８９．８±６．６％；胎盼蓝拒染存活率为８０．２±５．４％；粒一单系造血祖细胞（ＣＦＵ一ＧＭ）回收率为７１．１±８．２％。说明冻存效果好，ＤＭＳＯ用量减少，为ＰＢＳＣ的冷冻提供了一种简单而有效的方法。
his paper presents a simplified and inexpensive method for
cryopreservation of peri-pheral blood stem cells(PBSCs).6%hydroxyethyl starch(HES) and
5%dimethylsulfoxide(DMSO)were used as a cryoprotective agent at一80℃ without
rate-controlled freezing andliquid nitrogen。 PBSCs were stored in small polyprolene tubes for
one week to three nionths。After three months of cryopreservation, the nucleated cell
recoveries were 89.8±6.6%;try-pan blue viabilities and CFU-GM recoveries were 83.8±
6.0%and 71.1±8.2%,respective-ly.Those results indicated that this simplified method produced
high PBSCs recover rates。
