摘要
目的:探讨外源性碱性成纤维细胞生长因子(bFGF)在伤口愈合过程中可能的愈合机制以及对瘢痕形成的影响。方法:选用成年健康新西兰兔24只,建立兔耳创面愈合模型96个,随机分为bFGF治疗组和磷酸盐缓冲液(PBS)对照组,观察平均愈合时间、瘢痕体积、组织学变化及超微结构。结果:①bFGF组与对照组愈合时间分别为(18．4±1．6)d、(21．3±1．4)d,两组之间有显著性差异(P＜0．05)。②bFGF组瘢痕体积明显小于对照组,两组间差异有统计学意义(P＜0．01)。③光镜观察,bFGF组在愈合期炎症反应、血管形成及成纤维细胞增殖更明显。在愈合后的瘢痕组织中,胶原纤维排列较规则。对照组炎症反应时间较长,胶原纤维较粗大,排列紊乱。④电镜下可见治疗组成纤维细胞胶原合成持续时间较短。肥大细胞与成纤维细胞紧密接触,调整愈合全过程。后期表皮与真皮间有部分基底膜重建;对照组成纤维细胞合成持续时间较长,未见有基底膜的重建。结论:在伤口愈合过程中,外源性bFGF通过对细胞的调控有效地发挥促愈合作用;同时,通过部分重建基底膜结构,改善瘢痕的形成。
Objective:To investigate the effects of basic fibroblast growth factor (bFGF) on wound healing and scar formation and the underlying mechanism in rabbit.Methods:Ninety-six wounds were produced on adult rab- bit ears,and they were randomly divided into the bFGF treated group (group A) and the PBS control group(group B).The process of wound healing and the changes in scar formation were observed morphologically and histologi- cally.The mean time of wound healing and the size of scars were analyzed.Furthermore,samples of the repaired tissue from wounds were harvested for study under transmission electron microscope.Results:①The mean wound healing time of group A and B was (18.4±1.6)days and (21.3±1.4)days respectively.②The size of scars in group A was much smaller than that of group B.③Comparing with PBS controls,bFGF-treated wounds exhibited more obvious inflammatory response,angiogenesis and fibroblast proliferation during the process of wound heal- ing.However the collagen fiber appeared more slender and more regularly arranged after healing.④The follow- ing pictures were demonstrated by transmission electron microscopy.In group A,collagen synthesis time was short.Mast cells took an important role in the process.The basement membrane was re-constructed.Conclusion: The study indicates that the bFGF can effectively promote wound healing by its physiological functions.Mean- while,bFGF can improve scar quality by partly reconstructing the basal membrane.
出处
《感染．炎症．修复》
2006年第4期206-209,257,共5页
Infection Inflammation Repair
基金
广东省自然科学基金资助项目(04000570)
