莪术油注射液对小鼠移植性S180肉瘤血管形成的抑制作用

Experimental study on inhibiting angiogenesis in mice S180 sarcoma by curcuma phaeocaulis valeton

目的探讨莪术油对肿瘤血管形成抑制作用。方法通过建立动物肿瘤模型,运用莪术油予以实验治疗,结合免疫组化,检测其抑瘤率和肿瘤内血管密度,以及瘤体VEGF和bFGF表达的改变。结果莪术油具有明显的抑制S180肉瘤作用,其大剂量组抑瘤率达46.68%。小、中、大剂量实验组的S180瘤体内微血管密度均明显低于对照组;免疫组化显示莪术油大剂量组均可抑制S180瘤体内VEGF,bFGF的表达。结论莪术油对小鼠S180肉瘤有一定的抑制作用,对肿瘤血管形成均有明显抑制作用,降低VEGF、bFGF的表达可能是其抑制肿瘤血管形成的主要机制之一。

Objective To investigate the effect of inhibiting angiogenesis by curcuma phaeocaulis valeton. Methods The tumor inhibitory rate and the vascular density were tested in animal tumor model with experimental treatment, The expression of VEGF and bFGF were measured by immunohistochemistry, Results When the high dose was used, the tumor inhibitory rate of the curcuma phaeocaulis valeton were 46.68 %. The vascular density of S180 sarcoma were lower than the control group, and the expression of VEGF bFGF were down-regulated when the middle and high dose were used. Conclusions Curcuma phaeocaulis valeton have inhibitory effect on S180 sarcoma and strong inhibitory effects on angiogenesis. Its mechanism may be assiociated with the down-regulating of VEGF and bFGF expression.