双歧杆菌增殖因子的筛选及培养基的优化

The Bifidobacteria′s Growth-Promoting Factors Selection and the Basic Medium Optimization

研究了各种增殖因子对双歧杆菌的增殖效果,选出几种增殖效果好、价格低的物质进行正交试验优化培养基.得出长双歧杆菌(PBA)增殖培养基:在基础培养基基础上额外按体积分数添加平菇汁20%、卷心菜汁20%、豆浆20%、胡萝卜汁5%.在此培养基中,PBA经16 h培养,菌数可达2.45×109CFU/mL;得出婴儿双歧杆菌(INF)增殖培养基:在基础培养基基础上额外按体积分数添加平菇汁10%,蕃茄汁15%,肝浸汁15%,在此培养基中INF经16 h培养,菌数可达2.58×109CFU/mL.

Bifidobacteria are benefieal to human health. This bacteria require higher nutrition and the bifidus factors as the growth factor. The effect of different enrichment factors on bifidobacteria growth was studied. Several enrichment factors, good for bifidobacteria growth and with lower price, were selected to optimize the enrichment cultures. The resulting PBA medium was then obtained： adding the following substance into the basic medium 20° mushroom juice,20% savoy juice, 20% soybean-milk and 5% carrot juice. Under this condition the viable cell amount in the PBA could reach 2. 45×10^9 ;Another resulting medium INF was also obtained by： adding the following materials to basic medium.. 10 % mushroom juice, 15 % tomato juice, and 15% liver infusion, under this condition Viable cell amounts could reach 2.58×10^9 CFU/mL.