灰霉菌培养及其对拟南芥的侵染

The culture of Botrytis cinerea and its infection on Arabidopsis

灰葡萄孢菌Botrytis cinerea简称灰霉菌在以下条件生长发育最快:马铃薯+葡萄糖培养基,偏酸性pH 5～6、18～26 ℃温度,每天给予8 h的光照.Botrytis cinerea与拟南芥Columbia生态型有亲和反应.显微镜下观察到病原菌在拟南芥的Columbia 生态型上感染后1d可以在表皮细胞壁处层积、萌发和产生萌发管.第二天产生附着胞和侵入锥.第三天叶肉细胞间菌丝形成,叶肉细胞有损伤.第四天可观察到大量的菌丝,此时拟南芥的叶片产生可见病症.6～7d分生孢子梗和分生孢子产生.对可见和显微症状的观察说明寄主与病原菌之间发生亲和反应.

The optimal condition of gray mold Botrytis cinerea in experiment were potato agar medium plus 1.5 % dextrose at 18- 26℃ and 8 hours light per day. When inoculated to Columbia,an ecotype of Arabidopsis thaliana, it could be microscopically observed that the pathogen conidia moved over epidermal cell walls,germinated and developed germinating tuber in the first day, then appressoria and invasion pricks formed next day, the hyphae could be seen between mesophyllic cells into third day,abundant hyphae and visible symptoms in Columbia leaves could be observed 4 days after inoculation, and in the sixth or seventh day,the conidiophores were developed and conidia were reproduced.Observation to the macroscopical and microscopical process indicated that the host and pathogen were compatible.