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转录调节因子LMO4在牙胚发育中的基因表达 被引量:2

Gene expression of transcription regulator LMO4 in tooth morphogenesis
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摘要 目的观察转录调节因子LMO4在鼠磨牙牙胚形态发生中的基因表达,并与Shh信号分子的基因表达进行比较.方法制备昆明小鼠磨牙形态发育各期标本(E11.5~P1.5), whole-mount 原位杂交分析 LMO4 mRNA 在鼠胚中的表达与分布.切片原位杂交分析LMO4 及 Shh mRNA 在牙胚中的表达与分布.用免疫组化SP法对增殖细胞核抗原(PCNA)进行定位研究.结果 whole-mount 原位杂交发现,在E11.5, LMO4 mRNA在上下颌突、肢芽、脑、表皮和体节中呈阳性表达.切片原位杂交发现, E13.5~E16.5, LMO4 mRNA 分别在牙蕾上皮、成釉器两侧尖部和颈环处呈阳性表达,Shh mRNA表达于釉结; E18.5~P1.5, LMO4 mRNA 在成釉细胞层和中间层呈阳性表达.免疫组化染色发现,E13.5~E16.5,部分牙蕾上皮及颈环处细胞PCNA阳性,恰与LMO4基因表达部位重合.结论 LMO4在牙胚形态发生中呈时空特异性表达并且局限表达于上皮来源的组织.LMO4与Shh信号分子表达范围邻近,在牙胚发育早期可能调控细胞增殖,晚期可能参与成釉细胞的分化. Objective To investigate the expression of transcription regulator LMO4 mRNA in the developing mouse molar and compare the expression pattern of LMO4 with that of Shh signaling molecule. Methods Wild-type embryos used in this study ( E11.5 - P1.5 ) were generated by mating Kun-Ming mice. The expression pattern of LMO4 during organ development was carried on by whole-mount in situ hybridization. The expression patterns of LMO4 and Shh mRNA during molar development were analysed by section in situ hybridization. Immunohistochemical staining of PCNA was carried on by SP method. Results LMO4 mRNA was widespread at early embryonic stages ( E11.5 ) with positive hybridization signal in the mandibular reagon, limb bud, brain, epidermis and somites revealed by whole-mount in situ hybridization. Section in situ hybridization showed that LMO4 was expressed in the tooth bud, the two tips of the enamel organ and the cervical loop from E13.5 to E16. 5. While Shh was localized in the enamel knot on El4. 5. On E18. 5- P1.5, LMO4 transcripts were distributed in the ameloblast and the stratum intermedium. On E13.5 - E16. 5, the tooth bud cells and the cervical loop cells were PCNA positive. These were the same regions that showed LMO4 mRNA expression. Conclusions LMO4 was confined to the dental epithelium and had spatialtemporal expression patterns during tooth morphogenesis. The expression patterns of LMO4 and Shh were similar. In early tooth development, LMO4 might regulate cell proliferation. In late tooth development,it might participate in the ameloblast differentiation.
出处 《中华口腔医学杂志》 CAS CSCD 北大核心 2005年第5期398-401,共4页 Chinese Journal of Stomatology
基金 国家自然科学基金资助项目(30171011)
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参考文献10

  • 1Retaux S, Bachy I. A short history of LIM domains (1993-2002) :from protein interaction to degradation. Mol Neurobiol, 2002,26:269-281.
  • 2Kenny DA, Jurata LW, Saga Y, et al. Identification and characterization of LMO4, an LMO gene with a novel pattern of expression during embryogenesis. Proc Natl Acad Sci U S A, 1998,95 : 11257-11262.
  • 3Bulchand S, Subramanian L, Tole S. Dynamic spatiotemporal expression of LIM genes and cofactors in the embryonic and postnatal cerebral cortex. Dev Dyn, 2003, 226:460-469.
  • 4Coboume MT, Sharpe PT. Tooth and jaw: molecular mechanisms of patterning in the first branchial arch. Arch Oral Biol, 2003, 48:1-14.
  • 5Coboume MT, Miletich I, Sharpe PT. Restriction of sonic hedgehog signalling during early tooth development. Development , 2004,131 :2875-2885.
  • 6Hardcastle Z, Hui CC, Sharpe PT. The Shh signalling pathway in early tooth development. Cell Mol Biol, 1999, 45:567-578.
  • 7Chen Y, Dong D, Kostetskii I, et al. Henson's node from vitamin A-deficient quail embryo induces chick limb bud duplication and retainsits normal asymmetric expression of Sonic hedgehog (Shh). Dev Biol, 1996,173:256-264.
  • 8Zhang Y, Zhao X, Hu Y, et al. Msxl is required for the induction of Patched by Sonic hedgehog in the mammalian tooth germ. DevDyn, 1999 , 215:45-53.
  • 9陈智,张露,王志峰,孙志军,张旗,范兵.Shh及其受体Ptc1、Ptc2在鼠帽状期磨牙的基因表达[J].中华口腔医学杂志,2003,38(2):93-95. 被引量:10
  • 10Gritli-Linde A, Bei M, Maas R, et al. Shh signaling within the dental epithelium is necessary for cell proliferation, growth and polarization. Development, 2002, 129:5323-5337.

二级参考文献11

  • 1Jernvall J;Aberg T;Kettunen P.The life history of an embryonic signaling center:BMP-4 induces p21 and is associated with apoptosis in the mouse tooth enamel knot,1998.
  • 2Hammerschmidt M;Brook A;McMahon AP.The world according to hedgehog[J],1997(1).
  • 3Dassule HR;Lewis P;Bei M.Sonic hedgehog regulates growth and morphogenesis of the tooth[J],2000.
  • 4Hardcastle Z;Hui CC;Sharpe PT.The Shh signalling pathway in early tooth development[J],1999(45).
  • 5Motoyama J;Heng H;Crackower MA.Overlapping and non-overlapping Ptch2 expression with Shh during mouse embryogenesis[J],1998.
  • 6Pelton RW;Dickinson ME;Moses HL.In situ hybridization analysis of TGF beta 3 RNA expression during mouse development:comparative studies with TGF beta 1 and beta 2,1990.
  • 7Jernvall J;Thesleff I.Retune of lost structure in the developmental control of tooth shape,2000.
  • 8Yu CC;Woods AL;Levision DA.The assessment of cellular proliferation by immunohistochemistry: a review of currently available methods and their applications[J],1992.
  • 9Jernvall J;Thesleff I.Reiterative signaling and patterning during mammalian tooth morphogenesis[J],2000.
  • 10Fraidenraich D;Iwahori A;Rudnicki M.Activation of fgf4 gene expression in the myotomes is regulated by myogenic bHLH factors and by sonic hedgehog[J],2000.

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  • 1Grier RL 4th, Wise GE. Inhibition of tooth eruption in the rat by a bisphosphonate. J Dent Res, 1998,77(1 ) :8-15.
  • 2Tsurukai T, Udagawa N, Matsuzaki K, et al. Roles of macrophage-colony stimulating factor and osteoclast differentiation factor in osteoclastogenesis. J Bone Miner Metab 2000,18 (4) :177-184.
  • 3Wise GE, Lumpkin SJ, Huang H, et al. Osteoprotegerin and osteoclast differentiation factor in tooth eruption. J Dent Res, 2000, 79(12) :1937-1942.
  • 4Horewitz MC, Xi Y, Wilson K, et al. Control of osteoclastogenesis and bone resorption by members of the TNF family of receptors and ligands. Cytokine Growth Factor Rev, 2001, 12( 1 ) :9-18.
  • 5Yasuda H, Shima N, Nakagawa N, et al, Osteoclast differentiation factor is a ligand for osteoprotegerin/osteoclastogenesis-inhibitory factor and is identical to TRANCE/RANKL Proc Natl Acad Sci U S A, 1998,95(7) :3597-3602.
  • 6Odgren PR, Kim N, MacKay CA, et al. The role of RANKL ( TRANCE/TNF-SF11 ) , a tumor necrosis factor family member, in skeletal development: effects of knockout and transgenic rescue. Connect Tissue Res, 2003,44 Suppl 1:264-271.
  • 7Simonet WS, Lacey DL, Dustan CR, et al. Osteoprotegerin : a novel secreted protein involved in the regulation of bone density. Cell, 1997,89(2) :309-319.
  • 8Ohazama A, Courtney JM, Sharpe PT. OPG, RANK, and RANKL in tooth development: co-ordination of odontogenesis and osteogenesis. J Dent Res, 2004,83(3) :241-244.
  • 9Heinrich J, Bsoul S, Barnes J, et al. CSF-1, RANKL and OPG regulate ostelclastogenesis during murine tooth eruption. Arch Oral Biol, 2005,50(10) :897-908.
  • 10Volejnikova S, Laskari M, Marks SC Jr, et al. Monocyte recruitment and expression of monocyte chemoattractant pretein-1 are developmentally regulated in remodeling bone in the mouse. Am J Pathol, 1997,150(5) :1711-1721.

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