丹参与山莨菪碱对犬急性出血坏死性胰腺炎致肺早期损伤保护作用的研究

Protective Effects of Salviae miltiorrhizae and Anisodamine(654-2)Against Early Lung Injury in Acute Hemorrhagic Necrotizing Pancreatitis of Dog

本实验用体重7.5～11kg 犬18只,随机分成4组,用5%牛磺胆酸钠1 ml/kg逆行胰管注射制作 AHNP 模型,然后分别给予丹参(5g/Kg)、654-2(5mg/kg)和盐水静脉滴注。发现 AHNP 组早期 PaO_2、PaCO_2、pH 无明显改变,支气管肺泡灌洗浪 LDH、白蛋白、LPO 明显高于丹参组(P<0.05),透射电镜下见肺血管内皮细胞坏死,连接中断和血管壁缺损,而丹参组肺血管内皮细胞和肺Ⅱ型上皮细胞正常,提示丹参具有保护肺毛细血管内皮细胞和肺Ⅱ型上皮细胞作用,可能是一种氧自由基的清除剂;654-2有保护细胞膜、减少白细胞溶酶体酶释放的作用,对急性出血坏死性胰腺炎致肺早期损伤有一定的保护作用。

Experimental study had been conducted on 18 healthy adult mongral dogs of both sex,weighting from 7.5～11 kg,randomly divided into four groups.Experimental models of acute hemorrhagic necrotizing pancreatitis(AHNP)were established by retrograde injecting i ml/kg of sodium tauro- cholate directly into the pancreatic duct.The dogs were treated respectively with intravenous infusion of Salviae miltiorrhizae(5 g/kg),654-2(5 mg/kg)or normal saline.The results showed that P_aO_2,P_aCO_2 and pH did not change in early stage of AHNP.The contents of lactic acid dehy- drogenase(LDH),albumin and lipid peroxide(LPO)of bronchoalveolar lavage fluid in the AHNP group were significantly higher than that of Salviae miltiorrhizae group(P<0.05).The necrosis and disruption of conjunction of endotheliai cells resulting from the defects of vascular wall were noted under transmission electron microscope.Both pulmonary vascular and type Ⅱ pneumocyte were normal in the Salviae miltiorrhizae group.These results suggested that Salviae miltiorrhizae possess the effect of protecting endothelial cells of pulmonary vascular and type Ⅱ pneumocyte,which could function as scavenger of oxygen-derived free radicals.