rmOP-1对肾脏缺血再灌注损伤的预防与治疗作用

PREVENTIVE AND THERAPEUTICAL EFFECTS OF rmOP-1 ON RENAL ISCHEMIA-REPERFUSION INJURY

①目的探讨重组骨形成蛋白-1(rmOP-1)对Wistar大鼠肾脏缺血再灌注损伤的预防与治疗作用.②方法建立Wistar大鼠肾脏缺血再灌注模型,随机分为预防组、治疗组和对照组,前两组经肠系膜上静脉注射rmOP-1,剂量为250 μg/kg体质量,对照组给予等量生理盐水,分别检测各组大鼠血液中尿素氮(BUN)、肌酐(Cr)浓度,观察肾脏组织学变化并进行改良Miller评分.③结果预防组与治疗组血BUN、Cr值和Miller评分差异无显著性(F=17.47～78.15,q=1.05～2.50,P>0.05),但均明显低于对照组(t=4.39～11.18,P<0.01),组织学观察显示前两组大鼠肾小管上皮细胞坏死程度明显减轻.④结论 rmOP-1能够有效地保护缺血再灌注肾脏的结构和功能,为临床应用提供了可靠的理论基础.

Objective To investigate the preventive and therapeutical effects of rmOP-1 on kidneys with ischemia-reperfusion injury. Methods Thirty Wistar rat models of renal ischemia-reperfusion were randomly divided into three groups： preventive group, therapeutic group and control group. The first two groups were administered withrmOP-1, 250μg/kg bw, through the superior mesenteric vein. An equal-quantity normal saline was given to the control group. BUN and Cr were measured and renal histology assessed with modified Miller＇s scoring. Results The difference between preventive and therapeutic groups was not significant in terms of serum BUN and Cr and Miller＇s scoring （F=17.47-78. 15, q=1. 05-2.50, P〉0. 05）, but was markedly lower than that of the control group （t=4.39-11.18, P〈0.01）. Histology showed that rmOP-1 decreased the renal tubule cell necrosis. Conclusion rmOP-1 can effectively protect the function and structure of kidneys with ischemia-reperfusion injury, which provides a theoretical basis for clinical application.