摘要
用网格搜索寻优法确定了分离6种青霉素类药物的薄层色谱溶剂系统。采用均匀设计法安排初始实验,借助计算机进行网格搜索寻优计算,在硅胶GF(254)板上成功地分离了青霉素钠、苯唑青霉素钠、磺苄青霉素钠、氨苄青霉素钠、氧哌嗪青霉素钠和羧苄青霉素钠,方法简便、有效。
This paper presents the grid search procedure as a simple and efficient optimization method for the selection of thin-layer chromatography solvent system. The relationship between R_f and the proportions, X_1,X_2,X_3, ...,X_(n-1),of more polar solvents in developer can be expressed as ln(1 /R_f - 1 ) =b_0+b_1 lnX_1+b_2,lnX_2 +b_3lnX_3 +. +b_(n_1),lnX_(n-1),with X-1 + X_2 + X_3 + ... + X_(n-1) + X_n= 1, where X_n is the proportion of the least polar solvent. Two chromatographic optimization functions (COF) are used to judge the separation quality. (1)If the separation of all peaks is equally desirable, the COF is defined as When |△Rfi|>0.1,|△Rfi|is set equal to 0.1 ;(2)If one or more separations of peak pair is more important than the others,the COF is defined as. Where ωi is the weighting factor for the ith pair of peaks.The algorithm is programmed on a micro-computer.With the data of uniform design experiment,computer simulation is carried out and finally the optimum solvent systems are located. The above procedure was successfully applied to the separation of 6 sorts of penicillin drugs on silica layers. The optimum solvent compositions were chloroform-acetone-ethanol-formic acid of 0.64:0.09:0.16:0.11 and of 0.58:0.23:0.09:0.10. Plates were exposed to iodine vapor and scanned immediately after being enclosed. Measurements were made in the reflectance mode at the wavelength of 380nm.
出处
《色谱》
CAS
CSCD
北大核心
1996年第3期211-213,共3页
Chinese Journal of Chromatography
关键词
薄层色谱
溶剂系统
青霉素
thin-layer chromatography, optimzation of solvent systems, penicillins