摘要
利用自制的酶柱反应器,通过改变反应液流量、溶剂的种类、反应时间以及水含量等参数,考察了大豆油和甲醇在固定化脂肪酶(Cand ida sp.99-125)催化下进行酯交换反应制备生物柴油的工艺条件,并用气相色谱对产物进行了分析。实验结果表明,反应液流量和水含量对转化率影响比较大,转化率随反应液流量的增加出现先增后减的趋势,采用正己烷为溶剂要优于其它溶剂。当以固定化的脂肪酶为催化剂、正己烷为溶剂、n(大豆油)∶n(甲醇)=1∶3、m(大豆油)∶m(水)=5∶1、反应时间为24h、反应液流量1.2mL/m in时,产物中主要脂肪酸甲酯的质量分数可以达到91.87%。
An enzyme -column reactor was developed to carry out transesterification of soybean oil and methanol on immobilized lipase catalyst （ Candida sp. 99 - 125 ）. Main influencing factors to reaction were flow rate of reactant, varieties of solvents, water content and reaction time. Among them flow rate of reactant and water content were the two more important factors. Transesterification conversion first increased and then decreased with flow rate of reactant, n - Hexane was the best in five kinds of solvents tested. Under optimal conditions ： mole ratio of soybean oil to methanol 1 - 3, solvent n - hexane, mass ratio of soybean oil to water 5 ： 1, reaction time 24 h and flow rate of reaction liquid 1.2 mL/min, primary fatty acid methyl esters in product reached 91.87%.
出处
《石油化工》
EI
CAS
CSCD
北大核心
2005年第9期855-858,共4页
Petrochemical Technology
基金
广州市科技计划项目(2004J1-C0101)
关键词
生物柴油
固定化
脂肪酶
催化
酯交换反应
大豆油
甲醇
正己烷
bio - diesel oil
immobilize
lipase
catalyst
transesterification
soybean oil
methanol
n - hexane
