摘要
以海藻酸钠(SA)和聚乙烯醇(PVA)为包埋材料,采用固定化细胞技术对纳豆菌生产纳豆激酶进行了研究。研究发现,在PVA中加入SA进行细胞包埋可获得渗透性能好强度高的固定化细胞,通过正交试验进一步确定:当PVA的浓度为11%,SA的浓度为1%,硼酸的浓度为5%,CaC l2的浓度为6%时,固定化细胞的强度最好,可反复使用6批次,活性也很高,产生的纳豆激酶酶活溶纤圈直径积达88mm2/15μL。
The immobilizing technology ofBacillus Natto cells is studied by taking Alginate Calcium and Polyvinyl Alcohol as entrapping agents. It is discovered that the immobilized cells of SA and PVA are stronger than those of SA. When the immobilized cells are made by PVA 11%, ,SA 1% ,boric acid 5% ,Calcium chloride 6% ,they have both of good mechanical stability and mass transfer ability .It can be used six times in repeatedly. The fibrinolytic activity of immobilized cells are also very strong.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2005年第4期363-366,共4页
Journal of Nanchang University(Natural Science)
关键词
固定化细胞
海藻酸钠
聚乙烯醇
包埋剂
immobilized cells
alginate calcium
polyvinyl alcohol
entrapping agents
