头穴透刺对急性脑梗塞大鼠血浆中cAMP与-βEP含量的影响

Effect of Electroacupuncture of Head Acupoints on the Contents of Serum cAMP and β-EP in Rats with Acute Cerebral Infarction

目的:探讨头穴透刺对急性脑梗塞大鼠血浆中cAMP(环磷酸腺苷)与-βEP(β内啡肽)含量变化的影响。方法:健康SD大鼠90只,按随机数字表法分为正常组10只,模型组40只,头穴透刺组40只。模型组与头穴透刺组分6 h2、4 h、48 h、72 h 4个时相分别取材,每小组10只。用线栓法直接阻断大鼠大脑中动脉开口处制作急性脑梗塞模型,用“百会”透“前顶”“率谷”透“悬厘”针刺治疗。采用放免技术检测各组大鼠血浆中cAMP与-βEP的含量。结果:造模后,急性脑梗塞大鼠血浆中cAMP含量明显低于正常组,血浆中-βEP含量明显高于正常组。头穴透刺治疗可使大鼠血浆中cAMP的含量明显升高,使-βEP含量明显降低而趋于正常值。结论:头穴透刺可能通过升高血浆中过低的cAMP含量和降低异常升高的-βEP含量而达到治疗急性脑梗塞的目的。

Objective： To research the effect of electroacupuncture （EA） of head acupoints on the contents of serum cAMP and β-EP in rats with acute cerebral infarction （CI）. Methods： Ninety SD rats were randomized into normal group （10 rats）, model group （40 rats） and EA group （40 rats） according to random number table. Rats of model and EA groups were respectively and evenly divided into 6 h, 24 h, 48 h, and 72 h subgroups. Acute CI model was pro（teed by occlusion of the right middle cerebral artery. After penetration needling from “Baihui” （ GV 20） to “Qianding” （ GV 21, i.e. MS 5）, and “Shuaigu” （ 8） to “Xuanli” （GB 6）, EA （strength 1-2 mA, frequency 30 Hz, continuous waves） was applied for 30 min, once every 12 hours for 24 h, 48 h and 72 h subgroups. Blood samples were taken separately for assaying the contents of serum cAMP and β-EP with radioimmunoessay （RIA）. Results： In comparison with normal group, serum cAMP levels of all the subgroups of model group, and 6 h and 24 h subgroups of EA group lowered remarkably （ P〈0.05, 0.01 ）, while β-EP levels of all the subgroups of model group, and 6 h and 24 h subgroups of EA group increased significantly （P〈0.05, 0.01 ）. Compared with subgroups of model group, serum cAMP content of 72 h subgroup of EA group was significantly higher （ P〈 0.05）, while β-EP contents of 24 h, 48 h and72 h subgroups of EA group decreased considerably （P〈0.05, 0.01）. It indicated that after CI, serum cAMP level decreased markedly, while serum β-EP level increased obviously; after EA, both serum cAMP and β-EP levels turned to normal gradually. Conclusion： EA of head acupoints can suppress CI-induced abnomlal decrease of serum cAMP content and increase of β-EP level, which may contribute to the favorable effect of EA in treating acute cerebral infarction.