重组人表皮生长因子促进大鼠皮肤创面愈合的研究

A study on the promoting effects of recombinant human epidermal growth factor on skin wound healing in rats

目的观察重组人表皮生长因子(rhEGF)对皮肤创面愈合的作用。方法制作大鼠背部创伤模型,采用自身平行对照,将34只大鼠背部的68个创面分成rhEGF治疗组与盐水对照组,观察大体形态和组织学改变、创面愈合时间和愈合率,测定伤后不同时间创面羟脯氨酸(OHP)含量和Ⅰ型Ⅲ型胶原比例,进行细胞DNA周期分析。结果经rhEGF治疗的创面愈合速度较盐水对照明显加快,2组平均愈合时间为(17.2±1.3)d和(20.5±1.6)d(P<0.01);外用rhEGF使创面肉芽组织生成增多,再上皮化明显,显著增加创面中OHP含量,降低Ⅰ型Ⅲ型胶原比例,加速细胞DNA复制。结论外用rhEGF可缩短创面愈合时间,增加肉芽组织及OHP含量,降低Ⅰ型Ⅲ型胶原比例,加速细胞DNA复制,明显促进皮肤创面的修复。

Objective To observe the effects of recombinant human epidermal growth factor （rhEGF） on skin wound healing. Methods The dorsal trauma model of rats was ttsed. A total of 68 dorsal wounds in 34 rats were created and divided into tile rhEGF group and the isotonic saline group according to self-control. The process of wound healing was observed and a mean healing time was calculated. Furthermore, the dynamic analysis was performed at different times on wound OHP contents, ratio of collagen Ⅰ /Ⅲ and cell DNA cycle. Results The wound healing was accelerated obviously in all wounds treated with rhEGF. The mean time of wound healing of the two groups was（ 17.2 ± 1.3） days and （20.5 ± 1.6） days respeetively （ P 〈 0.01）. In the rhEGF group, the new granulation tissue was more and the re-epithelialization was faster than that of the saline group. External rhEGF increased OHP content, reduced collageo Ⅰ /Ⅲ ratio and accelerated cell DNA replication. Conclusion External rhEGF ean shorten wound healing time, inerease grannlation tissue and OHP contents, reduce collagen Ⅰ /Ⅲ ratio and accelerate cell DNA replication, thus obviously promoting skin wound repair.