摘要
目的研究红大戟快速繁殖技术,为人工栽培提供种源。方法以野生红大戟的茎尖作外植体,通过不同的培养基进行诱导培养获得丛生芽,进而生根获得再生植株。结果M S+BA 2.0 m g/L+NAA 0.1 m g/L适合红大戟的丛生芽继代增殖。1/2M S+NAA 1.0 m g/L适宜诱导生根获得健全再生植株。移栽成活率70%。结论本研究得出的方法可为人工栽培红大戟提供种源。
Objective The tissue culture of the stem apex of Knoxia valerianoides was done to research the optimum culture medium for test-tube seedlings for large-scale planting. Methods The stem apex of wild K. valerianoides was used as the explants and cultivated in different culture media separately to induce fascicled-bud, root, and plantlet regeneration. Results The cultural medium MS＋BA 2.0 mg/ L＋NAA 0.1 mg/L was suitable for the sub-multiplication of fascicled bud and the medium 1/2MS＋NAA 1. 0 mg/L for root inducement and the plantlet regeneration. The transplantation survival rate of the plantlet regeneration was 70%. Conclusion The tissue culture of K. valerianoides could be used to produce test-tube seedlings for large-scale planting.
出处
《中草药》
CAS
CSCD
北大核心
2005年第10期1555-1557,共3页
Chinese Traditional and Herbal Drugs
基金
广西壮族自治区科技厅科技攻关项目(0322024-3A)
关键词
红大戟
组织培养
试管苗
Knoxia valerianoides Thorel et Pitard
tissue culture
test-tube seedling
