组织工程化软骨形成的细胞浓度选择

Selection of optimal cell density of tissue engineered cartilage

目的探讨兔组织工程化软骨形成的合适细胞浓度。方法分离培养兔关节软骨细胞,体外扩增后以1,2,4,6,8,10×107/ml 6种不同浓度种植到PLGA支架上。在体外培养软骨细胞支架复合物,4周终止培养,进行HE、Masson组织学染色、Ⅱ型胶原免疫组织化学染色。结果体外培养的软骨组织,与正常软骨组织有相似的组织学特性,形成软骨组织适宜细胞浓度为4×107/ml。结论体外形成兔软骨组织的适宜细胞接种浓度为4×107/ml。

Objective To investigate the suitable selection of cell density of tissue engineered cartilage. Methods The chondrocytes isolated from rabbit articular cartilage were seeded onto PLGA scaffold after expansion by in vitro culture, and the densities of cell suspensions were 1,2,4,6,8,10 × 10^7/ml. After 4 weeks of culture, the complex was assessed by histological staining. Immunohistochemical analysis was performed to evaluate collagen type Ⅱ synthesis. Results Articular chondrocytes were well distributed and adhered to PLGA scaffolds and their characteristics of articular cartilage were kept throughout the culture period. The optimal chondrocyte density is 4× 10^7/ml. Conclusion With tissue engineering skills,an optimal chondrocvte densitv of 4 × 10^7/ml is suggested.