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用AFLP分子标记鉴定冬枣自然授粉实生后代杂种的研究 被引量:36

Application of AFLP Markers for Identification of Hybrids from Open Pollinated Dongzao(Zizyphus jujuba Mill.) Progenies
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摘要 应用AFLP技术对65株冬枣自然授粉实生后代进行杂种鉴定,从81对EcoRⅠ和M seⅠ选择性引物中筛选出12对带型清晰且多态性较高的引物。用这12对引物共扩增出517条谱带,其中多态性带376条,多态性百分率为72.7%。共扩增出金丝小枣特征带10条,鉴定出34株冬枣×金丝小枣杂交实生苗;扩增出尖枣特征带7条,鉴定出冬枣×尖枣杂交实生苗15株;不能确定的有16株。 The 65 open-pollinated ‘Dongzao' ( Zizyphus jujuba Mill. ) progenies were detected with AFLP technique. Twelve pairs of primers which amplified distinct and polymorphic segments were selected from 81 pairs of EcoR Ⅰand Mse Ⅰprimers. Then twelve pairs of primers were used in the AFLP analysis.Altogether 517 bands were produced among which 376 bands were polymorphic ones with the percentage of polymorphism being 72. 7%. Ten markers were present only in ‘Jinsi Xiaozao'. Thirty-four hybrids from ‘Dongzao' בJinsi Xiaozao' were identificated. Seven markers were present only in ‘Jianzao' Fifteen hybrids from ‘Dongzao' בJianzao' were identificated. Sixteen plants were not confirmed.
出处 《园艺学报》 CAS CSCD 北大核心 2005年第4期680-683,共4页 Acta Horticulturae Sinica
基金 国家自然科学基金项目(30370994)
关键词 实生后代 AFLP标记 杂种鉴定 Chinese jujube Progeny AFLP marker Identification of hybrid
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  • 1Paterson A, Brubaker C, Wendel J. A rapid method for extraction of(Gossypium spp)genomic DNA suitable for RFLP or PCR analysis. Plant Molecular Biology Reporter, 1993, 11(2): 122~127.
  • 2Vos P R, Hogers M. Bleeker. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res.,1995, 23 (21): 4407~4414.
  • 3张开春,李荣旗,毕晓颖,尹淑萍,吴禄平,景士西.RAPD技术鉴定无融合生殖型平邑甜茶的有性后代[J].农业生物技术学报,1997,5(4):392-396. 被引量:28

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