生长激素预处理保护大鼠肝脏缺血再灌注损伤的研究

Effect of growth hormone on hepatic ischemia-reperfusion injury in rats

目的:探讨重组人生长激素(rhGH)预处理对肝脏缺血再灌注损伤的保护作用及其可能机制。方法:16只雄性W istar大鼠随机等分为rhGH组和对照组,分别用rhGH或盐水预处理后以Pringle法阻断入肝血流15m in,于再灌注1 h末测定血清AST、ALT、LDH水平,肝组织谷胱甘肽(GSH)与超氧化物歧化酶(SOD)水平以及肝组织病理学改变。结果:rhGH组再灌注后1 h血清AST、ALT、LDH水平显著低于对照组(P<0.05)。rhGH组肝组织GSH、SOD水平均显著高于对照组(P<0.05)。rhGH组肝组织损伤程度明显轻于对照组。结论:生长激素预处理通过提高肝组织中GSH、SOD水平减轻大鼠肝脏缺血再灌注损伤。

Objective： To investigate the protective effect of recombinant human growth hormone（rhGH） on hepatic ischemia-reperfusion injury（HIRI） in rats. Methods：Sixteen male Wistar rats were randomly divided into rhGH group and control group. Hepatic inflow was occluded for 15 minutes by Pringle＇ s maneuver to establish HIPI rat model after the rats were preconditioned with growth hormone or saline. The concentrations of AST, ALT, and LDH in the serum, the concentrations of GSH and SOD in liver tissue,and histopathological change of liver were determined respectively 1hour after reperfusion. Results-One hour after re perfusion, serum concentrations of AST, ALT, and LDH in＇rhGH group were significandy lower than those in control group（ P 〈 0.05） ;levels of GSH and SOD in rhGH group were significantly higher than those in control group（ P 〈0.05） ;the histopathological change of liver in rhGH group was obviously slighter than that in control group. Concluslon：Preconditioning of rhGH may alleviate HIRI by increasing the content of GSH and SOD in liver tissue of rats.